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Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus

Wohlres-Viana, S ; Arashiro, E K N ; Machado, M A ; Camargo, L S A ; Siqueira, L G B ; Palhao, M P ; Viana, J H M

Reproduction fertility and development, 2017-09, Vol.29 (10), p.1958-1970 [Periódico revisado por pares]

Australia

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  • Título:
    Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus
  • Autor: Wohlres-Viana, S ; Arashiro, E K N ; Machado, M A ; Camargo, L S A ; Siqueira, L G B ; Palhao, M P ; Viana, J H M
  • Assuntos: Alternative Splicing ; Animals ; Cattle ; Estradiol - biosynthesis ; Female ; Follicular Fluid - metabolism ; Gene Expression ; Ovarian Follicle - metabolism ; Progesterone - biosynthesis ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Receptors, LH - genetics ; Receptors, LH - metabolism
  • É parte de: Reproduction fertility and development, 2017-09, Vol.29 (10), p.1958-1970
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The aim of the present study was to characterise the roles of intrafollicular oestradiol production and granulosa cell (GC) expression of the LH receptor (LHR) gene and its isoforms during follicular deviation in Bos indicus. Follicular wave emergence was synchronised in heifers from a Bos taurus dairy (Holstein; n=10) and a B. indicus dairy breed (Gir; n=10). Follicles were aspirated individually at sizes corresponding to the periods of predeviation, deviation and postdeviation. Intrafollicular oestradiol (IF-E2) and progesterone (IF-P4) concentrations were determined in the follicular fluid (FF) by radioimmunoassay, and relative expression of P450 aromatase (CYP19A1) and LHR forms was evaluated in GC using real-time quantitative-polymerase chain reaction. Despite differences in the size of the dominant follicle at deviation, changes in CYP19A1 expression and IF-E2 concentrations were similar in follicles of the same diameter in both breeds. A peak in total LHR expression occurred after follicular deviation in association with low expression of LHR isoforms. The results suggest that regulation of LHR function by sequential changes in the expression pattern of LHR isoforms may play a role in the early deviation of the dominant follicle, as observed in B. indicus breeds.
  • Editor: Australia
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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