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Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes

Pereira, Michele M ; Machado, Marco A ; Costa, Fernanda Q ; Serapiao, Raquel V ; Viana, Joao H.M ; Camargo, Luiz S.A

Reproduction fertility and development, 2010, Vol.22 (7), p.1074-1082 [Periódico revisado por pares]

Australia: Collingwood, Victoria: CSIRO Publishing

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Título:
Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes
Autor: Pereira, Michele M ; Machado, Marco A ; Costa, Fernanda Q ; Serapiao, Raquel V ; Viana, Joao H.M ; Camargo, Luiz S.A
Assuntos: Animals ; apoptosis ; Apoptosis - physiology ; blastocyst ; Blastocyst - physiology ; Cattle - genetics ; Cattle - physiology ; Culture Media - pharmacology ; Female ; Fertilization in Vitro - methods ; Fertilization in Vitro - veterinary ; Gene Expression Profiling - veterinary ; In Situ Nick-End Labeling - veterinary ; Male ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - physiology ; Oxygen - administration & dosage ; Polyvinyl Alcohol - pharmacology ; Random Allocation ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA - chemistry ; RNA - genetics ; Serum
É parte de: Reproduction fertility and development, 2010, Vol.22 (7), p.1074-1082
Notas: http://dx.doi.org/10.1071/RD10007
ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: With an aim to improve the in vitro production of bovine embryos, the present study investigated the effect of serum and oxygen tension during IVM on oocyte developmental competence. Four experimental groups were evaluated: G1, 10% oestrus cow serum (OCS) with 20% O₂; G2, 0.1% polyvinyl alcohol (PVA) with 20% O₂; G3, 10% OCS with 5% O₂; and G4, 0.1% PVA with 5% O₂. The proportion of MII oocytes, blastocyst rates and total cell number were not affected (P > 0.05) when the OCS was replaced with PVA under 5% O₂, whereas a higher (P 0.05) were found in maturation and blastocyst rates. Significant differences were found in the amount of specific transcripts in oocytes matured under different conditions. In conclusion maturation with PVA and 5% O₂ provides an efficient in vitro culture condition for the maturation of bovine oocytes.
Editor: Australia: Collingwood, Victoria: CSIRO Publishing
Idioma: Inglês

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Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes

Pereira, Michele M ; Machado, Marco A ; Costa, Fernanda Q ; Serapiao, Raquel V ; Viana, Joao H.M ; Camargo, Luiz S.A

Australia: Collingwood, Victoria: CSIRO Publishing

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