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Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

Damous, Luciana Lamarão ; Nakamuta, Juliana Sanajotti ; Soares, Jr, José Maria ; Maciel, Gustavo Arantes Rosa ; Simões, Ricardo Dos Santos ; Montero, Edna Frasson de Souza ; Krieger, José Eduardo ; Baracat, Edmund Chada

Journal of ovarian research, 2014-03, Vol.7 (1), p.34-34 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function
Autor: Damous, Luciana Lamarão ; Nakamuta, Juliana Sanajotti ; Soares, Jr, José Maria ; Maciel, Gustavo Arantes Rosa ; Simões, Ricardo Dos Santos ; Montero, Edna Frasson de Souza ; Krieger, José Eduardo ; Baracat, Edmund Chada
Assuntos: Analysis ; Animals ; Apoptosis ; Caspase 3 - metabolism ; Estrous Cycle ; Experiments ; Female ; Females ; Fertility ; Glucose ; Graft Survival ; Gynecology ; Heart ; Hypoxia ; Hypoxia - metabolism ; Hypoxia - pathology ; Hypoxia - physiopathology ; Laboratory animals ; Medical research ; Methods ; Ovarian Follicle - metabolism ; Ovarian Follicle - pathology ; Ovarian Follicle - physiopathology ; Ovarian Follicle - transplantation ; Ovariectomy ; Ovary - metabolism ; Ovary - pathology ; Ovary - physiopathology ; Ovary - transplantation ; Ovulation ; Oxygen - metabolism ; Rats, Wistar ; Recovery of Function ; Rodents ; Studies ; Time Factors ; Tissue Culture Techniques ; Tissue Preservation - methods ; Tissue Survival ; Transplantation of organs, tissues, etc ; Transplants & implants
É parte de: Journal of ovarian research, 2014-03, Vol.7 (1), p.34-34
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Descrição: Cryopreservation of the ovarian tissue has shown promising results. However, there remain controversial issues such as the short half-life of grafts. In this aspect, there are some evidences that preconditioning the ovarian tissue before transplantation is beneficial. To determine the effect of hypoxic preconditioning in vitro on ovarian tissue prior to transplantation. Eighteen female adult Wistar rats, were sorted into three experimental groups. Ovaries were maintained in DMEM low glucose serum free at 37°C with 5% CO2, at atmospheric oxigen concentration (normoxia) or 1% O2 (hypoxia) for 16 hours. Oxigen concentration was determined by injection of nitrogen in the incubator. Animals submitted to ovarian transplantation immediately after oophorectomy were the Control Group (C). After this, the ovaries were implanted in the retroperitoneum with nonabsorbable suture and animals evaluated for thirty days after transplantation. Beginning on postoperative (PO) day 11, a daily collection of vaginal smear was carried out. Analyses comprised morphological, morphometric (counting ovarian follicles and corpora lutea) and immunohistochemistry for cleaved caspase-3 (apoptosis). In normoxia and control groups all animals recovered their estrous cycles, while in the hypoxia group, two animals did not ovulate but, among those which did, resumption took longer than in the other groups (p < 0.05). The number of ovarian follicles and corpora lutea decreased significantly in the hypoxia group when compared to the other two groups (p < 0.001) and apoptosis was increased in the few ovarian follicles which remained viable (p < 0.001). The hypoxic preconditioning in vitro was not beneficial to the graft and worsened their viability, compromising its functionality or delaying the return of this.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

Damous, Luciana Lamarão ; Nakamuta, Juliana Sanajotti ; Soares, Jr, José Maria ; Maciel, Gustavo Arantes Rosa ; Simões, Ricardo Dos Santos ; Montero, Edna Frasson de Souza ; Krieger, José Eduardo ; Baracat, Edmund Chada

England: BioMed Central Ltd

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