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Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells

Ito, Mikiko ; Sakai, Yuko ; Furumoto, Mari ; Segawa, Hiroko ; Haito, Sakiko ; Yamanaka, Setsuko ; Nakamura, Rie ; Kuwahata, Masashi ; Miyamoto, Ken-ichi

American journal of physiology: endocrinology and metabolism, 2005-06, Vol.288 (6), p.E1101-E1109 [Periódico revisado por pares]

United States

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Título:
Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells
Autor: Ito, Mikiko ; Sakai, Yuko ; Furumoto, Mari ; Segawa, Hiroko ; Haito, Sakiko ; Yamanaka, Setsuko ; Nakamura, Rie ; Kuwahata, Masashi ; Miyamoto, Ken-ichi
Assuntos: Animals ; Base Sequence ; Calcitriol - metabolism ; Calcitriol - pharmacology ; Cloning, Molecular ; DNA - chemistry ; DNA - genetics ; Fibroblast Growth Factors - chemistry ; Fibroblast Growth Factors - genetics ; Fibroblast Growth Factors - metabolism ; Humans ; K562 Cells ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Molecular Sequence Data ; Phosphates - metabolism ; Phosphates - pharmacology ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Receptors, Calcitriol - metabolism ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Vitamin D Response Element - physiology
É parte de: American journal of physiology: endocrinology and metabolism, 2005-06, Vol.288 (6), p.E1101-E1109
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Department of Molecular Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima City, Japan Submitted 22 October 2004 ; accepted in final form 20 January 2005 Fibroblast growth factor-23 (FGF-23) has been recently identified as playing an important pathophysiological role in phosphate homeostasis and vitamin D metabolism. To elucidate the precise physiological regulation of FGF-23, we characterized the mouse FGF-23 5'-flanking region and analyzed its promoter activity. The 5'-flanking region of the mouse FGF-23 gene contained a TFIID site (TATA box) and several putative transcription factor binding sites, including MZF1, GATA-1 and c-Ets-1 motifs, but it did not contain the typical sequences of the vitamin D response element. Plasmids encoding 554-bp (pGL/0.6), 364-bp (pGL/0.4) and 200-bp (pGL/0.13) promoter regions containing the TFIID element and +1-bp fragments drove the downstream expression of a luciferase reporter gene in transfection assays. We also found that FGF-23 mRNA was expressed in K-562 erythroleukemia cell lines but not in MC3T3-E1, Raji, or Hep G2 human carcinoma cells. Treatment with 1,25-dihydroxyvitamin D 3 in the presence of high phosphate markedly stimulated pGL/0.6 activity, but calcium had no effect. In addition, the plasma FGF-23 levels were affected by the dietary and plasma inorganic phosphate concentrations. Finally, the levels of plasma FGF-23 in vitamin D receptor-null mice were significantly lower than in wild-type mice. The presents study demonstrated that vitamin D and the plasma phosphate level are important regulators of the transcription of the mouse FGF-23 gene. gene regulation; vitamin D receptor; phosphate homeostasis Address for reprint requests and other correspondence: Ken-ichi Miyamoto, Institute of Health Biosciences, The Univ. of Tokushima Graduate School, Kuramoto-cho 318-15, Tokushima, Japan (E-mail: miyamoto{at}nutr.med.tokushima-u.ac.jp )
Editor: United States
Idioma: Inglês

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Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells

Ito, Mikiko ; Sakai, Yuko ; Furumoto, Mari ; Segawa, Hiroko ; Haito, Sakiko ; Yamanaka, Setsuko ; Nakamura, Rie ; Kuwahata, Masashi ; Miyamoto, Ken-ichi

United States

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