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Time course of traumatic neuroma development

Oliveira, Karla M C ; Pindur, Lukas ; Han, Zhihua ; Bhavsar, Mit B ; Barker, John H ; Leppik, Liudmila Blum, Robert

PloS one, 2018-07, Vol.13 (7), p.e0200548-e0200548 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Time course of traumatic neuroma development
Autor: Oliveira, Karla M C ; Pindur, Lukas ; Han, Zhihua ; Bhavsar, Mit B ; Barker, John H ; Leppik, Liudmila
Blum, Robert
Assuntos: Amputation ; Axonogenesis ; Axons ; Biology ; Biology and Life Sciences ; Care and treatment ; Complications and side effects ; Development and progression ; Developmental stages ; Eye injuries ; Fibrosis ; Histology ; Injuries ; Medical research ; Medicine ; Medicine and Health Sciences ; Morphology ; Muscles ; Nerves ; Nervous system tumors ; Neurofilament protein ; Neurofilaments ; Neurosciences ; Pain ; Physical characteristics ; Proteins ; Regrowth ; Smooth muscle ; Surgery ; Tumors
É parte de: PloS one, 2018-07, Vol.13 (7), p.e0200548-e0200548
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: This study was designed to characterize morphologic stages during neuroma development post amputation with an eye toward developing better treatment strategies that intervene before neuromas are fully formed. Right forelimbs of 30 Sprague Dawley rats were amputated and limb stumps were collected at 3, 7, 28, 60 and 90 Days Post Amputation (DPA). Morphology of newly formed nerves and neuromas were assessed via general histology and neurofilament protein antibody staining. Analysis revealed six morphological characteristics during nerve and neuroma development; 1) normal nerve, 2) degenerating axons, 3) axonal sprouts, 4) unorganized bundles of axons, 5) unorganized axon growth into muscles, and 6) unorganized axon growth into fibrotic tissue (neuroma). At early stages (3 & 7 DPA) after amputation, normal nerves could be identified throughout the limb stump and small areas of axonal sprouts were present near the site of injury. Signs of degenerating axons were evident from 7 to 90 DPA. From day 28 on, variability of nerve characteristics with signs of unorganized axon growth into muscle and fibrotic tissue and neuroma formation became visible in multiple areas of stump tissue. These pathological features became more evident on days 60 and 90. At 90 DPA frank neuroma formation was present in all stump tissue. By following nerve regrowth and neuroma formation after amputation we were able to identify 6 separate histological stages of nerve regrowth and neuroma development. Axonal regrowth was observed as early as 3 DPA and signs of unorganized axonal growth and neuroma formation were evident by 28 DPA. Based on these observations we speculate that neuroma treatment and or prevention strategies might be more successful if targeted at the initial stages of development and not after 28 DPA.
Editor: United States: Public Library of Science
Idioma: Inglês

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Time course of traumatic neuroma development

Oliveira, Karla M C ; Pindur, Lukas ; Han, Zhihua ; Bhavsar, Mit B ; Barker, John H ; Leppik, Liudmila Blum, Robert

United States: Public Library of Science

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