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Leptin Induces Oxidative Stress Through Activation of NADPH Oxidase in Renal Tubular Cells: Antioxidant Effect of L-Carnitine

Blanca, Antonio J. ; Ruiz-Armenta, María V. ; Zambrano, Sonia ; Salsoso, Rocío ; Miguel-Carrasco, José L. ; Fortuño, Ana ; Revilla, Elisa ; Mate, Alfonso ; Vázquez, Carmen M.

Journal of cellular biochemistry, 2016-10, Vol.117 (10), p.2281-2288 [Periódico revisado por pares]

United States: Blackwell Publishing Ltd

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Título:
Leptin Induces Oxidative Stress Through Activation of NADPH Oxidase in Renal Tubular Cells: Antioxidant Effect of L-Carnitine
Autor: Blanca, Antonio J. ; Ruiz-Armenta, María V. ; Zambrano, Sonia ; Salsoso, Rocío ; Miguel-Carrasco, José L. ; Fortuño, Ana ; Revilla, Elisa ; Mate, Alfonso ; Vázquez, Carmen M.
Assuntos: Animals ; Antioxidants - pharmacology ; Blotting, Western ; Carnitine - pharmacology ; Cells, Cultured ; Enzyme Activation ; Humans ; Kidney ; Kidney Tubules, Proximal - drug effects ; Kidney Tubules, Proximal - metabolism ; Kidney Tubules, Proximal - pathology ; L-CARNITINE ; LEPTIN ; Leptin - toxicity ; Medicin och hälsovetenskap ; NADPH OXIDASE ; NADPH Oxidases - genetics ; NADPH Oxidases - metabolism ; OXIDATIVE STRESS ; Oxidative Stress - drug effects ; Protective Agents - pharmacology ; Rats ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Superoxides - metabolism
É parte de: Journal of cellular biochemistry, 2016-10, Vol.117 (10), p.2281-2288
Notas: Fundación Pública Andaluza para la Gestión de la Investigación en Salud de Sevilla - No. RED 07/0003/2010
Instituto de Salud Carlos III, Subdirección General de Evaluación y Fomento de la Investigación, PN de I+D+I 2008-2011 - No. PS09/01395
ark:/67375/WNG-12CBHBGS-H
ArticleID:JCB25526
istex:CFB7D308A03EA9BEE2D7C4FBC6F7EBA4B0A34188
Consejería de Salud, Junta de Andalucía - No. PI-0060/2012
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: ABSTRACT Leptin is a protein involved in the regulation of food intake and in the immune and inflammatory responses, among other functions. Evidences demonstrate that obesity is directly associated with high levels of leptin, suggesting that leptin may directly link obesity with the elevated cardiovascular and renal risk associated with increased body weight. Adverse effects of leptin include oxidative stress mediated by activation of NADPH oxidase. The aim of this study was to evaluate the effect of L‐carnitine (LC) in rat renal epithelial cells (NRK‐52E) exposed to leptin in order to generate a state of oxidative stress characteristic of obesity. Leptin increased superoxide anion (O2•−) generation from NADPH oxidase (via PI3 K/Akt pathway), NOX2 expression and nitrotyrosine levels. On the other hand, NOX4 expression and hydrogen peroxide (H2O2) levels diminished after leptin treatment. Furthermore, the expression of antioxidant enzymes, catalase, and superoxide dismutase, was altered by leptin, and an increase in the mRNA expression of pro‐inflammatory factors was also found in leptin‐treated cells. LC restored all changes induced by leptin to those levels found in untreated cells. In conclusion, stimulation of NRK‐52E cells with leptin induced a state of oxidative stress and inflammation that could be reversed by preincubation with LC. Interestingly, LC induced an upregulation of NOX4 and restored the release of its product, hydrogen peroxide, which suggests a protective role of NOX4 against leptin‐induced renal damage. J. Cell. Biochem. 117: 2281–2288, 2016. © 2016 Wiley Periodicals, Inc. Stimulation of NRK‐52E cells with leptin induced a state of oxidative stress and inflammation that could be reversed by preincubation with LC. Interestingly, LC induced an upregulation of NOX4 and restored the release of its product, hydrogen peroxide, which suggests a protective role of NOX4 against leptin‐induced renal damage.
Editor: United States: Blackwell Publishing Ltd
Idioma: Inglês

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Leptin Induces Oxidative Stress Through Activation of NADPH Oxidase in Renal Tubular Cells: Antioxidant Effect of L-Carnitine

Blanca, Antonio J. ; Ruiz-Armenta, María V. ; Zambrano, Sonia ; Salsoso, Rocío ; Miguel-Carrasco, José L. ; Fortuño, Ana ; Revilla, Elisa ; Mate, Alfonso ; Vázquez, Carmen M.

United States: Blackwell Publishing Ltd

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