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Cloning, gene structure and dietary regulation of the type-IIc Na/Pi cotransporter in the mouse kidney

Ohkido, I ; Segawa, H ; Yanagida, R ; Nakamura, M ; Miyamoto, K

Pflügers Archiv, 2003-04, Vol.446 (1), p.106-115 [Periódico revisado por pares]

Germany: Springer Nature B.V

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Título:
Cloning, gene structure and dietary regulation of the type-IIc Na/Pi cotransporter in the mouse kidney
Autor: Ohkido, I ; Segawa, H ; Yanagida, R ; Nakamura, M ; Miyamoto, K
Assuntos: Amino Acid Sequence ; Animals ; Cloning, Molecular ; Humans ; Immunohistochemistry ; Kidney Tubules, Proximal - chemistry ; Kidney Tubules, Proximal - metabolism ; Kidney Tubules, Proximal - ultrastructure ; Male ; Mice ; Microvilli - chemistry ; Microvilli - metabolism ; Molecular Sequence Data ; Oocytes - metabolism ; Opossums ; Phosphorus, Dietary - administration & dosage ; Plasmids ; Rats ; RNA, Complementary - metabolism ; Sodium-Phosphate Cotransporter Proteins ; Symporters - analysis ; Symporters - genetics ; Symporters - metabolism ; Tissue Distribution ; Transfection ; Xenopus
É parte de: Pflügers Archiv, 2003-04, Vol.446 (1), p.106-115
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: We have demonstrated previously that the type-IIc Na/Pi cotransporter is a growth-related renal Na/Pi cotransporter that is highly expressed in kidney of the weaning rat. In the present study, we investigated type-IIc Na/Pi cotransporter function further by cloning the mouse gene and characterizing the corresponding protein. The mouse type-IIc transporter amino acid sequence shows a high degree of similarity to the human (86%) and rat (95%) type-IIc Na/Pi-cotransporters. The mouse gene contained 14 exons and mapped to chromosome 2. The DNA sequence upstream from exon 1 is GC rich. The upstream region does not contain an apparent TATA box, but does contain two dietary Pi-responsive elements, which are potential binding sites for the transcription factor micro E3 (TFE3). Microinjection of mouse type-IIc cRNA into Xenopus oocytes demonstrated sodium-dependent Pi cotransport activity. The affinity for Pi was about 200 microM in 100 mM Na. Feeding adult mice fed a low-Pi diet increased the expression of type-IIc protein in the apical membrane of renal proximal tubular cells. Hybrid depletion studies suggested that the type-IIc transporter contributes to about 30% of Na/Pi cotransport in the kidney of adult mice fed a low-Pi diet. The present study suggests that the type-IIc Na/Pi cotransporter is a functional of renal Pi transporter in adult mice fed a low-Pi diet.
Editor: Germany: Springer Nature B.V
Idioma: Inglês

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Cloning, gene structure and dietary regulation of the type-IIc Na/Pi cotransporter in the mouse kidney

Ohkido, I ; Segawa, H ; Yanagida, R ; Nakamura, M ; Miyamoto, K

Germany: Springer Nature B.V

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