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Measurement of H-2 and Non-H-2 Antigens in the Mouse with the Footpad Swelling Test

Kon, Neal D ; Klein, Paul A

The Journal of immunology (1950), 1976-08, Vol.117 (2), p.413-415 [Periódico revisado por pares]

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Título:
Measurement of H-2 and Non-H-2 Antigens in the Mouse with the Footpad Swelling Test
Autor: Kon, Neal D ; Klein, Paul A
Assuntos: Animals ; Female ; Hindlimb - immunology ; Histocompatibility Antigens - analysis ; Immunization ; Isoantigens - analysis ; Methods ; Mice ; Mice, Inbred A ; Mice, Inbred C57BL ; Skin Transplantation ; Transplantation, Homologous
É parte de: The Journal of immunology (1950), 1976-08, Vol.117 (2), p.413-415
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The footpad swelling (FPS) test for delayed-type hypersensitivity in the mouse was evaluated for its ability to measure histocompatibility (H) antigens present on intact spleen cells or solubilized from a methylcholanthrene-(MCA) induced fibrosarcoma. Mice were immunized to H-antigens (H-2 or non-H-2) with skin allografts and challenged 14 days later with test antigens. Spleen cell-bound and solubilized H-antigens elicited strong and specific FPS reactions in allograft-immunized but not in unimmunized mice. The test could distinguish H-2 from non-H-2 antigens as well as D from KI region specificities of the H-2 gene complex. These experiments suggest that the FPS test is a reliable in vivo method for measuring both cell-bound and solubilized H-antigens in the mouse.
Editor: United States: Am Assoc Immnol
Idioma: Inglês

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