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Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Juhas, Mark ; Engelmayr, George C. ; Fontanella, Andrew N. ; Palmer, Gregory M. ; Bursac, Nenad

Proceedings of the National Academy of Sciences - PNAS, 2014-04, Vol.111 (15), p.5508-5513 [Periódico revisado por pares]

United States: National Academy of Sciences

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Título:
Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo
Autor: Juhas, Mark ; Engelmayr, George C. ; Fontanella, Andrew N. ; Palmer, Gregory M. ; Bursac, Nenad
Assuntos: Animals ; Architectural engineering ; Artificial satellites ; Bioengineering ; Biological Sciences ; Biomimetics - methods ; Blood vessels ; Calcium ; Cobra Cardiotoxin Proteins - toxicity ; Mice ; Mice, Nude ; Microvessels - growth & development ; Muscle Contraction - physiology ; Muscle Development - physiology ; Muscle tissues ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - growth & development ; Muscles ; Musculoskeletal system ; Rodents ; Signal transduction ; Skeletal muscle ; Skeletal muscle satellite cells ; Stem cells ; Tissue engineering ; Tissue Engineering - methods
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2014-04, Vol.111 (15), p.5508-5513
Notas: http://dx.doi.org/10.1073/pnas.1402723111
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Author contributions: M.J., G.C.E., and N.B. designed research; M.J. and G.C.E. performed research; M.J., A.N.F., and G.M.P. contributed new reagents/analytic tools; M.J. and N.B. analyzed data; and M.J. and N.B. wrote the paper.
Edited by Robert Langer, Massachusetts Institute of Technology, Cambridge, MA, and approved March 7, 2014 (received for review February 14, 2014)
Descrição: Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady ingrowth of blood-perfused microvasculature along with an increase in amplitude of calcium transients and force of contraction. We also demonstrated superior structural organization, vascularization, and contractile function of fully differentiated vs. undifferentiated engineered muscle implants. The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Juhas, Mark ; Engelmayr, George C. ; Fontanella, Andrew N. ; Palmer, Gregory M. ; Bursac, Nenad

United States: National Academy of Sciences

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