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Nitric oxide protocols

Michael A Titheradge

Totowa, N.J. Humana Press c1998

Localização: FMRP - Fac. Medicina de Ribeirão Preto    (578 N731 29143 ) e outros locais(Acessar)

  • Título:
    Nitric oxide protocols
  • Autor: Michael A Titheradge
  • Assuntos: Nitric oxide -- Metabolism; Nitric oxide -- Synthesis; TECNICAS BIOLOGICAS; Nitric Oxide -- isolation & purification -- Laboratory Manuals; Nitric Oxide -- metabolism -- Laboratory Manuals
  • Notas: Includes bibliographical references and index
  • Descrição: Enzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [p1sC]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne-- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer,
    Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Martín-Sanz and Lisardo Boscá -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- Apractical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- Use of NO donors in biological systems / Jayne M. Tul
  • Títulos relacionados: Série:Methods in molecular biology (Clifton, N.J.) v. 100
  • Editor: Totowa, N.J. Humana Press
  • Data de criação/publicação: c1998
  • Formato: xi, 324 p. ill 24 cm.
  • Idioma: Inglês

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