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DNA Barcoding Green Microalgae Isolated from Neotropical Inland Waters

Hadi, Sámed I I A ; Santana, Hugo ; Brunale, Patrícia P M ; Gomes, Taísa G ; Oliveira, Márcia D ; Matthiensen, Alexandre ; Oliveira, Marcos E C ; Silva, Flávia C P ; Brasil, Bruno S A F Kumar, Shashi

PloS one, 2016-02, Vol.11 (2), p.e0149284-e0149284 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
DNA Barcoding Green Microalgae Isolated from Neotropical Inland Waters
Autor: Hadi, Sámed I I A ; Santana, Hugo ; Brunale, Patrícia P M ; Gomes, Taísa G ; Oliveira, Márcia D ; Matthiensen, Alexandre ; Oliveira, Marcos E C ; Silva, Flávia C P ; Brasil, Bruno S A F
Kumar, Shashi
Assuntos: Algae ; Aquatic microorganisms ; Bar codes ; Biodiversity ; Biology and Life Sciences ; Brazil ; Chlorophyta ; Chlorophyta - classification ; Chlorophyta - genetics ; Computer and information sciences ; Deoxyribonucleic acid ; DNA ; DNA Barcoding, Taxonomic ; DNA, Plant ; DNA, Ribosomal Spacer ; Feasibility studies ; Gene amplification ; Gene sequencing ; Genetic Markers ; Identification ; Inland waters ; Inspection ; Markers ; Microalgae ; Microalgae - classification ; Microalgae - genetics ; Morphology ; Phylogeny ; Primers ; Research and Analysis Methods ; Sequence Analysis, DNA ; Taxonomy
É parte de: PloS one, 2016-02, Vol.11 (2), p.e0149284-e0149284
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: SIIAH BSAFB. Performed the experiments: SIIAH HS PPMB TGG MDO AM MECO. Analyzed the data: SIIAH BSAFB. Contributed reagents/materials/analysis tools: SFCP BSAFB. Wrote the paper: SIIAH HS FCPS BSAFB.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: This study evaluated the feasibility of using the Ribulose Bisphosphate Carboxylase Large subunit gene (rbcL) and the Internal Transcribed Spacers 1 and 2 of the nuclear rDNA (nuITS1 and nuITS2) markers for identifying a very diverse, albeit poorly known group, of green microalgae from neotropical inland waters. Fifty-one freshwater green microalgae strains isolated from Brazil, the largest biodiversity reservoir in the neotropics, were submitted to DNA barcoding. Currently available universal primers for ITS1-5.8S-ITS2 region amplification were sufficient to successfully amplify and sequence 47 (92%) of the samples. On the other hand, new sets of primers had to be designed for rbcL, which allowed 96% of the samples to be sequenced. Thirty-five percent of the strains could be unambiguously identified to the species level based either on nuITS1 or nuITS2 sequences' using barcode gap calculations. nuITS2 Compensatory Base Change (CBC) and ITS1-5.8S-ITS2 region phylogenetic analysis, together with morphological inspection, confirmed the identification accuracy. In contrast, only 6% of the strains could be assigned to the correct species based solely on rbcL sequences. In conclusion, the data presented here indicates that either nuITS1 or nuITS2 are useful markers for DNA barcoding of freshwater green microalgae, with advantage for nuITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker.
Editor: United States: Public Library of Science
Idioma: Inglês

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DNA Barcoding Green Microalgae Isolated from Neotropical Inland Waters

Hadi, Sámed I I A ; Santana, Hugo ; Brunale, Patrícia P M ; Gomes, Taísa G ; Oliveira, Márcia D ; Matthiensen, Alexandre ; Oliveira, Marcos E C ; Silva, Flávia C P ; Brasil, Bruno S A F Kumar, Shashi

United States: Public Library of Science

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