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Comparison of gene expression in individual preimplantation bovine embryos produced by in vitro fertilisation or somatic cell nuclear transfer

Camargo, L.S. de A ; Powell, A.M ; Vale Filho, V.R. do ; Wall, R.J

Reproduction fertility and development, 2005-01, Vol.17 (5), p.487-496 [Periódico revisado por pares]

Australia

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  • Título:
    Comparison of gene expression in individual preimplantation bovine embryos produced by in vitro fertilisation or somatic cell nuclear transfer
  • Autor: Camargo, L.S. de A ; Powell, A.M ; Vale Filho, V.R. do ; Wall, R.J
  • Assuntos: Animals ; Blastocyst - metabolism ; cattle ; Cattle - embryology ; cloning ; cloning (animals) ; Cloning, Organism ; early development ; Embryo Culture Techniques - veterinary ; embryogenesis ; Fertilization in Vitro - veterinary ; Fibroblasts - metabolism ; Gene Expression Profiling ; Glucose Transporter Type 5 - genetics ; in vitro fertilization ; L-Lactate Dehydrogenase - genetics ; Nuclear Transfer Techniques ; nuclear transplantation ; Octamer Transcription Factor-3 - genetics ; Polymerase Chain Reaction ; Sodium-Potassium-Exchanging ATPase - genetics
  • É parte de: Reproduction fertility and development, 2005-01, Vol.17 (5), p.487-496
  • Notas: Reproduction, Fertility and Development is an international journal publishing original research , review and comment in the fields of reproduction and developmental biology in humans, domestic animals and wildlife
    http://www.publish.csiro.au/nid/44.htm
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  • Descrição: In vitro fertilisation (IVF) and somatic cell nuclear transfer (SCNT) have been implicated in a variety of developmental abnormalities. Aberrant gene expression is likely to account for much of the diminished viability and developmental abnormalities observed. In the present study, the expression of multiple genes in IVF and SCNT bovine blastocyst-stage embryos were evaluated and compared with in vivo -produced embryos. Eleven genes expressed at and following maternal–zygotic transcription transition were evaluated in individual blastocysts by real-time polymerase chain reaction following RNA amplification. A subset of those genes was also evaluated in individual IVF and SCNT eight-cell embryos. A fibroblast-specific gene, expressed by nuclear donor cells, was also evaluated in IVF and SCNT embryos. The observed gene expression pattern at the eight-cell stage was not different between IVF and SCNT embryos ( P > 0.05). In vitro fertilisation and SCNT blastocyst expression was lower ( P < 0.01) for all genes compared with their in vivo -produced counterparts, except for lactate dehydrogenase isoenzyme A ( P < 0.001). The patterns of gene expression of the IVF and SCNT blastocysts were indistinguishable. Neither SCNT eight-cell nor blastocyst-stage embryos expressed the gene used as a fibroblast marker (collagen VIα1). For the genes evaluated, the level of expression was influenced more by the environment than by the method used to produce the embryos. These results support the notion that if developmental differences observed in IVF- and SCNT-produced fetuses and neonates are the result of aberrant gene expression during the preimplantation stage, those differences in expression are subtle.
  • Editor: Australia
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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