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Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification

Aonuma, Hiroka ; Suzuki, Moemi ; Iseki, Hiroshi ; Perera, Namal ; Nelson, Bryce ; Igarashi, Ikuo ; Yagi, Takeshi ; Kanuka, Hirotaka ; Fukumoto, Shinya

Biochemical and biophysical research communications, 2008-11, Vol.376 (4), p.671-676 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification
Autor: Aonuma, Hiroka ; Suzuki, Moemi ; Iseki, Hiroshi ; Perera, Namal ; Nelson, Bryce ; Igarashi, Ikuo ; Yagi, Takeshi ; Kanuka, Hirotaka ; Fukumoto, Shinya
Assuntos: Animals ; Anopheles - parasitology ; Anopheles stephensi ; Base Sequence ; Diagnosis ; DNA, Protozoan - analysis ; DNA, Protozoan - biosynthesis ; Female ; Insect Vectors - genetics ; LAMP ; Malaria ; Malaria - diagnosis ; Malaria - epidemiology ; Malaria - parasitology ; Male ; Molecular Sequence Data ; Mosquito ; Plasmodium ; Plasmodium berghei ; Plasmodium berghei - genetics ; Plasmodium berghei - isolation & purification ; Polymerase Chain Reaction - methods ; Protozoan Proteins - genetics ; Sensitivity and Specificity
É parte de: Biochemical and biophysical research communications, 2008-11, Vol.376 (4), p.671-676
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: With an aim to develop a quick and simple method to survey pathogen-transmitting vectors, LAMP (loop-mediated isothermal amplification) was applied to the identification of Plasmodium-carrying mosquitoes, specifically a Plasmodium-transmitting experimental model using rodent malaria parasite ( Plasmodium berghei) and anopheline mosquitoes ( Anopheles stephensi). The detection sensitivity limit of the LAMP reaction amplifying the SPECT2 gene was determined to be 1 × 10 2 purified Plasmodium parasites, estimated to be sufficient for reliable identification of infectious mosquitoes. The robustness of the LAMP reaction was revealed by its ability to detect both Plasmodium oocysts and sporozoites from an “all-in-one” template using whole mosquito bodies. Moreover, LAMP successfully identified an infectious mosquito carrying just a single oocyst in its midgut, a level that can be easily overlooked in conventional microscopic analysis. These observations suggest that LAMP is more reliable and useful for routine diagnosis of vector mosquitoes in regions where vector-borne diseases such as malaria are endemic.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification

Aonuma, Hiroka ; Suzuki, Moemi ; Iseki, Hiroshi ; Perera, Namal ; Nelson, Bryce ; Igarashi, Ikuo ; Yagi, Takeshi ; Kanuka, Hirotaka ; Fukumoto, Shinya

United States: Elsevier Inc

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