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Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly

Ibarlucea-Benitez, Itziar ; Ferro, Luke S ; Drubin, David G ; Barnes, Georjana

The Journal of cell biology, 2018-05, Vol.217 (5), p.1687-1700 [Periódico revisado por pares]

United States: Rockefeller University Press

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  • Título:
    Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly
  • Autor: Ibarlucea-Benitez, Itziar ; Ferro, Luke S ; Drubin, David G ; Barnes, Georjana
  • Assuntos: Anaphase ; Aurora B protein ; Cell division ; Chromosomes ; Contraction ; Cyclin-dependent kinase inhibitor p27 ; Cytogenetics ; Dismantling ; Kinases ; Microtubules ; Molecular modelling ; Passengers ; Proteins
  • É parte de: The Journal of cell biology, 2018-05, Vol.217 (5), p.1687-1700
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: Mitotic spindle disassembly after chromosome separation is as important as spindle assembly, yet the molecular mechanisms for spindle disassembly are unclear. In this study, we investigated how the chromosomal passenger complex (CPC), which contains the Aurora B kinase Ipl1, swiftly concentrates at the spindle midzone in late anaphase, and we researched the role of this dramatic relocalization during spindle disassembly. We showed that the kinesins Kip1 and Kip3 are essential for CPC relocalization. In cells lacking Kip1 and Kip3, spindle disassembly is severely delayed until after contraction of the cytokinetic ring. Purified Kip1 and Kip3 interact directly with the CPC and recruit it to microtubules in vitro, and single-molecule experiments showed that the CPC diffuses dynamically on microtubules but that diffusion stops when the CPC encounters a Kip1 molecule. We propose that Kip1 and Kip3 trap the CPC at the spindle midzone in late anaphase to ensure timely spindle disassembly.
  • Editor: United States: Rockefeller University Press
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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