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Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding

Adeniran, Adebiyi A. ; Fernández‐Santos, Nadia A. ; Rodríguez‐Rojas, Jorge J. ; Treviño‐Garza, Nancy ; Huerta‐Jiménez, Heron ; Mis‐Ávila, Pedro C. ; Pérez‐Pech, Wilbert A. ; Hernández‐Triana, Luis M. ; Rodríguez‐Pérez, Mario A.

Ecology and evolution, 2019-12, Vol.9 (23), p.13543-13554 [Periódico revisado por pares]

England: John Wiley & Sons, Inc

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  • Título:
    Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding
  • Autor: Adeniran, Adebiyi A. ; Fernández‐Santos, Nadia A. ; Rodríguez‐Rojas, Jorge J. ; Treviño‐Garza, Nancy ; Huerta‐Jiménez, Heron ; Mis‐Ávila, Pedro C. ; Pérez‐Pech, Wilbert A. ; Hernández‐Triana, Luis M. ; Rodríguez‐Pérez, Mario A.
  • Assuntos: CO1 gene ; Cutaneous leishmaniasis ; Cytochrome ; Cytochrome-c oxidase ; Cytochromes ; Deoxyribonucleic acid ; Disease control ; Disease transmission ; Divergence ; DNA ; DNA barcoding ; Flies ; Gene sequencing ; Health surveillance ; Identification ; Interspecific ; Leishmaniasis ; Library collections ; Mexico ; Mitochondria ; mitochondrial ; Morphology ; Original Research ; Parasitic diseases ; Public health ; sand flies ; Species ; Surveillance ; Taxonomy ; Vector-borne diseases ; Vectors
  • É parte de: Ecology and evolution, 2019-12, Vol.9 (23), p.13543-13554
  • Notas: Funding information
    Consejo Nacional de Ciencia Y Tecnología (CONACYT), Mexico, is acknowledged for the doctoral scholarship 291137/457158 granted to Adebiyi Abdulhakeem Adeniran and for grant numbers 271108, 280896, and 295569 to Mario A. Rodriguez‐Perez through MEXBOL‐CONACYT. We also acknowledge Instituto Politecnico Nacional grant SIP20181120. Luis M. Hernández‐Triana would like to thank the EU Framework Horizon 2020 Innovation Grant (EVAg, No. 653316) for funding. Publication fees were granted by COFAA‐IPN (No. 11422). The funders have no role in the design, collection, analysis, interpretation of data, and writing of the manuscript.
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    Adeniran and Fernández‐Santos contributed equally to this study.
  • Descrição: Leishmaniasis, a vector‐borne disease transmitted to humans through the bite of phlebotomine sand flies, is of public health significance in southeastern Mexico. Active and continuous monitoring of vectors is an important aspect of disease control for the prediction of potential outbreaks. Thus, the correct identification of vectors is paramount in this regard. In this study, we employed DNA barcoding as a tool for identifying phlebotomine sand flies collected in localized cutaneous leishmaniasis endemic areas of Quintana Roo, Mexico. Specimens were collected using CDC light and Shannon traps as part of the Mexican Ministry of Health surveillance program. DNA extraction was carried out using a nondestructive protocol, and morphological identification based on taxonomic keys was conducted on slide‐mounted specimens. Molecular taxonomic resolution using the 658‐bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was 100% congruent with the morphological identification. Seven species were identified: Lutzomyia cruciata (Coquillett 1907), Lutzomyia longipalpis (Lutz & Neiva 1912), Psathyromyia shannoni (Dyar 1929), Dampfomyia deleoni (Fairchild & Hertig 1947), Dampfomyia beltrani/steatopyga (Vargas & Díaz‐Nájera 1951), Bichromomyia olmeca olmeca (Vargas & Díaz‐Nájera, 1959), and Brumptomyia mesai (Sherlock 1962). Mean intraspecific divergence ranged from 0.12% to 1.22%, while interspecific distances ranged from 11.59% to 19.29%. Neighbor‐joining (NJ) analysis using the Kimura 2‐parameter model also showed specimens of the same species to be clustered together. The study provides the first cox1 sequences for three species of sand flies and indicates the utility of DNA barcoding for phlebotomine sand flies species identification in southeastern Mexico. Correct identification of vectors is paramount to control, and we employed the DNA barcoding as a tool for identifying phlebotomine sand flies collected in leishmaniasis endemic areas of Quintana Roo, Mexico. We provide the first cox1 sequences for three species of sand flies and indicate the utility of DNA barcoding for phlebotomine sand flies species identification in Mexico.
  • Editor: England: John Wiley & Sons, Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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