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Wide hybridization between Brazilian soybean cultivars and wild perennial relatives

Bodanese-Zanettini, M.H ; Lauxen, M.S ; Richter, S.N.C ; Cavalli-Molina, S ; Lange, C.E ; Wang, P.J ; Hu, C.Y. (Universidade Federal do Rio Grande do Sul, Porto Alegre (Brazil))

Theoretical and applied genetics, 1996-10, Vol.93 (5-6), p.703-709 [Periódico revisado por pares]

Heidelberg: Springer

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Título:
Wide hybridization between Brazilian soybean cultivars and wild perennial relatives
Autor: Bodanese-Zanettini, M.H ; Lauxen, M.S ; Richter, S.N.C ; Cavalli-Molina, S ; Lange, C.E ; Wang, P.J ; Hu, C.Y. (Universidade Federal do Rio Grande do Sul, Porto Alegre (Brazil))
Assuntos: Biological and medical sciences ; Classical genetics, quantitative genetics, hybrids ; CULTIVO DE EMBRIONES ; CULTURE D'EMBRYON ; EMBRYO CULTURE ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; GLYCINE MAX ; HIBRIDACION INTERESPECIFICA ; HYBRIDATION INTERSPECIFIQUE ; INTERSPECIFIC HYBRIDIZATION ; PLANTAS SILVESTRES ; PLANTE SAUVAGE ; Pteridophyta, spermatophyta ; Vegetals ; WILD PLANTS
É parte de: Theoretical and applied genetics, 1996-10, Vol.93 (5-6), p.703-709
Notas: F30
98B0505
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Employing a different culture strategy, we obtained a greatly improved frequency of embryo rescue in intersubgeneric soybean hybrids. Successful crosses were obtained in 31 different genotype combinations between nine Brazilian soybean lines as the female parents and 12 accessions from Glycine canescens, G. microphylla, G. tabacina and G. tomentella. The hybrid pod retention rate dropped to about 10% during the first 8 days after pollination and stayed largely unchanged up to the 20th day. Immature harvested seeds fell into three size groups: Group 1, smaller than 1.3 mm (mostly empty seed coats); Group 2, 1.9-5.0 mm; Group 3, larger than 5 mm (from selfing). A total of 90 putative hybrid embryos were rescued using a highly enriched B5 medium to nourish the newly dissected embryos. The growing embryos were then placed in a high osmotic, modified B5 medium to induce maturation and dormancy. Schenk and Hildebrandt medium was used to germinate the dormant, partially dehydrated, physiologically mature embryos. Approximately 37% of the rescued embryos developed into plantlets in vitro, and approximately 8% grew into mature plants in the greenhouse. Morphological, cytological and isoenzyme patterns confirmed the hybrid status of all seven mature plants, all of which were generated using G. tomentella G 9943 as the paternal parent. It was observed that all soybean lines crossed with G 9943 were capable of producing mature hybrid plants. There was no correlation between the initial size of Group 2 seeds and plant survival rate. The hybrids were cloned by grafting and treated with colchicine. One of the treated plants displayed chromosome doubling.
Editor: Heidelberg: Springer
Idioma: Inglês

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Wide hybridization between Brazilian soybean cultivars and wild perennial relatives

Bodanese-Zanettini, M.H ; Lauxen, M.S ; Richter, S.N.C ; Cavalli-Molina, S ; Lange, C.E ; Wang, P.J ; Hu, C.Y. (Universidade Federal do Rio Grande do Sul, Porto Alegre (Brazil))

Heidelberg: Springer

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