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Natural isotope correction improves analysis of protein modification dynamics

Dietze, Jörn ; van Pijkeren, Alienke ; Egger, Anna-Sophia ; Ziegler, Mathias ; Kwiatkowski, Marcel ; Heiland, Ines

Analytical and bioanalytical chemistry, 2021-12, Vol.413 (30), p.7333-7340 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Natural isotope correction improves analysis of protein modification dynamics
Autor: Dietze, Jörn ; van Pijkeren, Alienke ; Egger, Anna-Sophia ; Ziegler, Mathias ; Kwiatkowski, Marcel ; Heiland, Ines
Assuntos: Acetyl Coenzyme A - analysis ; Acetylation ; Analysis ; Analytical Chemistry ; Animals ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography, Liquid - methods ; Communication ; Dynamics ; Food Science ; HEK293 Cells ; Histones ; Humans ; Isotope Labeling - methods ; Isotopes ; Labelling ; Laboratory Medicine ; Low molecular weights ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Metabolomics ; Methods ; Mice ; Molecular Weight ; Monitoring/Environmental Analysis ; Post-translational modification ; Protein Processing, Post-Translational ; Proteins ; Proteins - chemistry ; Proteomics ; RAW 264.7 Cells ; Scientific imaging ; Spectroscopy ; Stable isotopes ; Tandem Mass Spectrometry - methods
É parte de: Analytical and bioanalytical chemistry, 2021-12, Vol.413 (30), p.7333-7340
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Stable isotope labelling in combination with high-resolution mass spectrometry approaches are increasingly used to analyze both metabolite and protein modification dynamics. To enable correct estimation of the resulting dynamics, it is critical to correct the measured values for naturally occurring stable isotopes, a process commonly called isotopologue correction or deconvolution. While the importance of isotopologue correction is well recognized in metabolomics, it has received far less attention in proteomics approaches. Although several tools exist that enable isotopologue correction of mass spectrometry data, the majority is tailored for the analysis of low molecular weight metabolites. We here present PICor which has been developed for isotopologue correction of complex isotope labelling experiments in proteomics or metabolomics and demonstrate the importance of appropriate correction for accurate determination of protein modifications dynamics, using histone acetylation as an example.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Natural isotope correction improves analysis of protein modification dynamics

Dietze, Jörn ; van Pijkeren, Alienke ; Egger, Anna-Sophia ; Ziegler, Mathias ; Kwiatkowski, Marcel ; Heiland, Ines

Berlin/Heidelberg: Springer Berlin Heidelberg

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