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Acute Regulation by Dietary Phosphate of the Sodium-Dependent Phosphate Transporter (NaP1-2) in Rat Kidney

Katai, Kanako ; Segawa, Hiroko ; Haga, Hiromi ; Morita, Kyoko ; Arai, Hidekazu ; Tatsumi, Sawako ; Taketani, Yutaka ; Miyamoto, Ken-ichi ; Hisano, Setsuji ; Fukui, Yoshihiro ; Takeda, Eiji

Journal of biochemistry (Tokyo), 1997-01, Vol.121 (1), p.50-55 [Periódico revisado por pares]

Oxford University Press

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  • Título:
    Acute Regulation by Dietary Phosphate of the Sodium-Dependent Phosphate Transporter (NaP1-2) in Rat Kidney
  • Autor: Katai, Kanako ; Segawa, Hiroko ; Haga, Hiromi ; Morita, Kyoko ; Arai, Hidekazu ; Tatsumi, Sawako ; Taketani, Yutaka ; Miyamoto, Ken-ichi ; Hisano, Setsuji ; Fukui, Yoshihiro ; Takeda, Eiji
  • Assuntos: diet ; kidney ; lysosome ; phosphate ; transporter
  • É parte de: Journal of biochemistry (Tokyo), 1997-01, Vol.121 (1), p.50-55
  • Notas: 1Supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan, and the Salt Science Research Foundation.
    istex:7E33C3790DFE0DE357AB6594C5626183A4032ADE
    ark:/67375/HXZ-RNXQ3WPR-9
    ArticleID:121.1.50
  • Descrição: Alteration of the dietary intake of phosphate (P1) leads to rapid changes in renal P1 transport activity. The present study, examined the underlying cellular mechanisms of the rapid regulation, with special reference to renal P1 cotransporter. Rats were fed either a low-P1 (0.02%) diet (CLP rats), the low-P1 diet followed by a high-P1 (1.2%) diet (AHP rats), or a normal (0.6%) diet (control rats). Na+-dependent P1 transport activity in the brush border membrane was significantly increased in CLP rats compared with control rats, and this activity decreased rapidly within 2 h after the change of diet in AHP rats. Kinetic analysis of P1 transport in the AHP rats indicated that the reduction was accompanied by a decrease in the apparent Vmax for Na+-dependent P1 uptake. Northern blot analysis showed no difference in the abundance of NaP1-2 mRNA of the kidney between AHP and CLP rats. In contrast, Western blot analysis of renal brush border membrane proteins of AHP rats indicated a significant decrease in the abundance of NaP1-2 protein as compared with CLP rats. Immunoreactive signals for NaP1-2 were detected in lysosomal fractions of AHP and CLP rats. Immunohistochemical analysis showed that, NaP1-2 immunoreactivity in AHP rats was largely reduced in the apical membrane of the proximal tubular epithelial cells. Neither cycloheximide nor actinomycin D affected high-P1-induced reduction of NaP1-2 protein in the brush border membrane of AHP rats, indicating that de novo protein synthesis of an unidentified regulator protein was not involved in the mechanism of this reduction. In contrast, treatment with colchicine, which disrupts microtubulers, abolished the effect of high-P1 diet on NaP1-2 expression. These results suggested that rapid endocytotic internalization of NaP1-2 may occur specifically in the brush border membrane following an acute increase in dietary P1 intake.
  • Editor: Oxford University Press
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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