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Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica

Marana, S. R. ; Terra, W. R. ; Cançado, F. C. ; Ferreira, C. ; Valério, A. A. ; Barbosa, J. A. R. G.

Acta crystallographica. Section F, Structural biology and crystallization communications, 2006-08, Vol.62 (8), p.750-752 [Periódico revisado por pares]

5 Abbey Square, Chester, Cheshire CH1 2HU, England: Blackwell Publishing Ltd

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Título:
Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica
Autor: Marana, S. R. ; Terra, W. R. ; Cançado, F. C. ; Ferreira, C. ; Valério, A. A. ; Barbosa, J. A. R. G.
Assuntos: AMMONIUM SULFATES ; Animals ; CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY ; CRYSTAL STRUCTURE ; CRYSTALLIZATION ; Crystallization Communications ; CRYSTALS ; DIFFUSION ; digestive lysozymes ; Digestive System - enzymology ; Houseflies - enzymology ; Isoenzymes - chemistry ; Isoenzymes - isolation & purification ; LYSOZYME ; Muramidase - chemistry ; Muramidase - isolation & purification ; Musca domestica ; PROPANOLS ; RESOLUTION ; SPACE GROUPS ; SYNCHROTRON RADIATION ; Synchrotrons ; X-RAY DIFFRACTION
É parte de: Acta crystallographica. Section F, Structural biology and crystallization communications, 2006-08, Vol.62 (8), p.750-752
Notas: ArticleID:AYF2LL5065
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ObjectType-Article-1
SourceType-Scholarly Journals-1
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These authors contributed equally to this work.
Descrição: Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting‐drop vapour‐diffusion method in the presence of ammonium sulfate or PEG/2‐propanol as the precipitant. X‐ray diffraction data were collected to a maximum resolution of 1.9 Å using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P21 (unit‐cell parameters a = 36.52, b = 79.44, c = 45.20 Å, β = 102.97°) and the orthorhombic space group P21212 (unit‐cell parameters a = 73.90, b = 96.40, c = 33.27 Å), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.
Editor: 5 Abbey Square, Chester, Cheshire CH1 2HU, England: Blackwell Publishing Ltd
Idioma: Inglês

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Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica

Marana, S. R. ; Terra, W. R. ; Cançado, F. C. ; Ferreira, C. ; Valério, A. A. ; Barbosa, J. A. R. G.

5 Abbey Square, Chester, Cheshire CH1 2HU, England: Blackwell Publishing Ltd

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