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Optimization of β-glucosidase, β-xylosidase and xylanase production by Colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification

Zimbardi, Ana L R L ; Sehn, Cesar ; Meleiro, Luana P ; Souza, Flavio H M ; Masui, Douglas C ; Nozawa, Monica S F ; Guimarães, Luis H S ; Jorge, João A ; Furriel, Rosa P M

International journal of molecular sciences, 2013-01, Vol.14 (2), p.2875-2902 [Periódico revisado por pares]

Switzerland: MDPI

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  • Título:
    Optimization of β-glucosidase, β-xylosidase and xylanase production by Colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification
  • Autor: Zimbardi, Ana L R L ; Sehn, Cesar ; Meleiro, Luana P ; Souza, Flavio H M ; Masui, Douglas C ; Nozawa, Monica S F ; Guimarães, Luis H S ; Jorge, João A ; Furriel, Rosa P M
  • Assuntos: Arachis hypogaea ; Colletotrichum graminicola ; Hypocrea jecorina ; Triticum aestivum
  • É parte de: International journal of molecular sciences, 2013-01, Vol.14 (2), p.2875-2902
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: Efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. The production of β-glucosidase, β-xylosidase and xylanase by Colletotrichum graminicola was optimized using Response Surface Methodology (RSM). Maximal production occurred in wheat bran. Sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and xylanase production, respectively. Maximal levels after optimization reached 159.3 ± 12.7 U g-1, 128.1 ± 6.4 U g-1 and 378.1 ± 23.3 U g-1, respectively, but the enzymes were produced simultaneously at good levels under culture conditions optimized for each one of them. Optima of pH and temperature were 5.0 and 65 °C for the three enzymes, which maintained full activity for 72 h at 50 °C and for 120 min at 60 °C (β-glucosidase) or 65 °C (β-xylosidase and xylanase). Mixed with Trichoderma reesei cellulases, C. graminicola crude extract hydrolyzed raw sugarcane trash with glucose yield of 33.1% after 48 h, demonstrating good potential to compose efficient cocktails for lignocellulosic materials hydrolysis.
  • Editor: Switzerland: MDPI
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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