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Induction of dendritic cell proliferation by neonate splenic adherent cells obtained by two different procedures

R V Laurentino K R B Bastos; A P F Do Rosário; C Zago; L R Sardinha; José Maria Alvarez; Maria Regina D'Império Lima; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

Item não circula. Consulte sua biblioteca.(Acessar)

  • Título:
    Induction of dendritic cell proliferation by neonate splenic adherent cells obtained by two different procedures
  • Autor: R V Laurentino
  • K R B Bastos; A P F Do Rosário; C Zago; L R Sardinha; José Maria Alvarez; Maria Regina D'Império Lima; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: IMUNOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and Objective: Dendritic cells (DCs) are crucial components of the immune system due to their essential role in T cell activation and differentiation. It is generally accept that DCs lose their capacity to proliferate after differentiation from its precursors. However, it has been recently shown that endothelial-like splenic stromal cells are able to induce the proliferation of fully differentiated and matured bone marrow-derived DCs (BMDCs) ( Nature Immunol. 2004; 5: 1124-33 ). Probably by mimicking the in vivo requirements, a close contact between both types of cells seems to be necessary to stimulate DC proliferation. Based on these findings, the main purpose of this study was to compare neonate splenic adherent cells (NSACs) obtained by two different procedures, regarding the ability to stimulate BMDC proliferation. Methods and Results: Adherent cells were obtained from the spleen of neonate C57BL/6 mice by two different procedures: 1) Pieces (1 mm2) of splenic tissue were cultured as previously described ( Nature Immunol. 2004; 5: 1124-33 ); and 2) Spleen cells were treated with trypsin and colagenase and let to adhere. After 3 weeks of culture, flow cytometry analysis revealed that NSACs obtained by procedures 1 and 2 expressed CD11b (68,39% and 8,50% ), CD106 (1,68% and 13,43%) and Thy-1 (9,77% and 14,73%), respectively. BMDCs were generated in the presence of rGM-CSF and rIL-4, as previously described (
    Nature Immunol. 2004; 5: 1124-33). These cells were stained with CFSE and cultured for 7 (CFSE fluorescence intensity from 824,50 to 32,20 and 35,77) or 14 days (CFSE fluorescence intensity from 824,50 to 20,15 and 22,75) in the presence of NSACs (at a ratio of 1:10 ) or alone. As indicated by the decrease of CFSE fluorescence intensity, BMDCs kept in contact with NSACs obtained by both procedures seem to proliferate at similar levels. In contrast, the absence of proliferation of BMDCs maintained alone was suggested by the fact that CFSE fluorescence intensity was similarly high before and after the period of culture (824,50 and 500,44). Conclusions: Our results suggest that NSACs obtained either from pieces of splenic tissue or from enzymes digested spleen can induce BMDC proliferation with similar efficiency.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. CI.012.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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