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Rat adipose tissue-derived stem cells transplantation attenuates cardiac dysfunction post infarction and biopolymers enhance cell retention

Danoviz, Maria E ; Nakamuta, Juliana S ; Marques, Fabio L N ; dos Santos, Leonardo ; Alvarenga, Erica C ; dos Santos, Alexandra A ; Antonio, Ednei L ; Schettert, Isolmar T ; Tucci, Paulo J ; Krieger, Jose E Schwartz, Arnold

PloS one, 2010-08, Vol.5 (8), p.e12077-e12077 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Rat adipose tissue-derived stem cells transplantation attenuates cardiac dysfunction post infarction and biopolymers enhance cell retention
Autor: Danoviz, Maria E ; Nakamuta, Juliana S ; Marques, Fabio L N ; dos Santos, Leonardo ; Alvarenga, Erica C ; dos Santos, Alexandra A ; Antonio, Ednei L ; Schettert, Isolmar T ; Tucci, Paulo J ; Krieger, Jose E
Schwartz, Arnold
Assuntos: Adipose tissue ; Adipose Tissue - cytology ; Adipose tissues ; Allografts ; Angiogenesis ; Animals ; Biopolymers ; Biopolymers - administration & dosage ; Biopolymers - pharmacology ; Biotechnology/Bioengineering ; Blood pressure ; Bone marrow ; Cardiology ; Cardiomyocytes ; Cardiovascular Disorders/Heart Failure ; Cardiovascular Disorders/Myocardial Infarction ; Cell adhesion & migration ; Cell Biology ; Cell body ; Cell culture ; Cell Survival - drug effects ; Cells, Cultured ; Cerebral infarction ; Coinjection ; Collagen ; Collagen - administration & dosage ; Collagen - pharmacology ; Coronary vessels ; Diastolic pressure ; Emission analysis ; Female ; Fibrin ; Fibrin - administration & dosage ; Fibrin - pharmacology ; Heart ; Heart - drug effects ; Heart - physiopathology ; Heart attack ; Heart attacks ; Heart diseases ; Heart failure ; Heart transplantation ; Hemodynamics ; Injection ; Injections ; Laboratory animals ; Measurement ; Medical schools ; Morphometry ; Myocardial infarction ; Myocardial Infarction - metabolism ; Myocardial Infarction - pathology ; Myocardial Infarction - physiopathology ; Myocardial Infarction - surgery ; Myocardium ; Myocardium - metabolism ; Myocardium - pathology ; Organs ; Ostomy ; Pharmacology ; Physiology/Cardiovascular Physiology and Circulation ; Preservation ; Radiation ; Radiation measurement ; Rats ; Rats, Inbred Lew ; Retention ; Rodents ; Stem Cell Transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism ; Stroke volume ; Syngeneic grafts ; Transplantation ; Veins & arteries ; Ventricle ; Viability
É parte de: PloS one, 2010-08, Vol.5 (8), p.e12077-e12077
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: MED JSN JEK. Performed the experiments: MED JSN FLM LdS ECA AAdS ELA ITS PJT. Analyzed the data: MED JSN LdS PJT. Contributed reagents/materials/analysis tools: LdS JEK. Wrote the paper: MED JSN JEK.
Descrição: Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI). 99mTc-labeled ASCs (1x10(6) cells) isolated from isogenic Lewis rats were injected 24 hours post-MI using fibrin a, collagen (ASC/C), or culture medium (ASC/M) as vehicle, and cell body distribution was assessed 24 hours later by gamma-emission counting of harvested organs. ASC/F and ASC/C groups retained significantly more cells in the myocardium than ASC/M (13.8+/-2.0 and 26.8+/-2.4% vs. 4.8+/-0.7%, respectively). Then, morphometric and direct cardiac functional parameters were evaluated 4 weeks post-MI cell injection. Left ventricle (LV) perimeter and percentage of interstitial collagen in the spare myocardium were significantly attenuated in all ASC-treated groups compared to the non-treated (NT) and control groups (culture medium, fibrin, or collagen alone). Direct hemodynamic assessment under pharmacological stress showed that stroke volume (SV) and left ventricle end-diastolic pressure were preserved in ASC-treated groups regardless of the vehicle used to deliver ASCs. Stroke work (SW), a global index of cardiac function, improved in ASC/M while it normalized when biopolymers were co-injected with ASCs. A positive correlation was observed between cardiac ASCs retention and preservation of SV and improvement in SW post-MI under hemodynamic stress. We provided direct evidence that intramyocardial injection of ASCs mitigates the negative cardiac remodeling and preserves ventricular function post-MI in rats and these beneficial effects can be further enhanced by administering co-injection of ASCs with biopolymers.
Editor: United States: Public Library of Science
Idioma: Inglês

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Rat adipose tissue-derived stem cells transplantation attenuates cardiac dysfunction post infarction and biopolymers enhance cell retention

Danoviz, Maria E ; Nakamuta, Juliana S ; Marques, Fabio L N ; dos Santos, Leonardo ; Alvarenga, Erica C ; dos Santos, Alexandra A ; Antonio, Ednei L ; Schettert, Isolmar T ; Tucci, Paulo J ; Krieger, Jose E Schwartz, Arnold

United States: Public Library of Science

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