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Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems

Bai, Yeran ; Camargo, Carolina M ; Glasauer, Stella M K ; Gifford, Raymond ; Tian, Xinran ; Longhini, Andrew P ; Kosik, Kenneth S

Nature communications, 2024-01, Vol.15 (1), p.350-350, Article 350 [Periódico revisado por pares]

England: Nature Publishing Group

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Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems
Autor: Bai, Yeran ; Camargo, Carolina M ; Glasauer, Stella M K ; Gifford, Raymond ; Tian, Xinran ; Longhini, Andrew P ; Kosik, Kenneth S
Assuntos: Astrocytes ; Azides ; Brain - diagnostic imaging ; Cell culture ; Cell differentiation ; Fluorescence ; Heterogeneity ; Humans ; Induced Pluripotent Stem Cells ; Infrared instruments ; Lipid metabolism ; Lipids ; Medical imaging ; Metabolism ; Metabolites ; Microglia ; Neuroimaging ; Organoids ; Pluripotency ; Stem cells ; Synthesis
É parte de: Nature communications, 2024-01, Vol.15 (1), p.350-350, Article 350
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Descrição: Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms of metabolic-related diseases. Current metabolic imaging studies suffer from limitations including low resolution and specificity, and the model systems utilized often lack human relevance. Here, we present a single-cell metabolic imaging platform to enable direct imaging of lipid metabolism with high specificity in various human-derived 2D and 3D culture systems. Through the incorporation of an azide-tagged infrared probe, selective detection of newly synthesized lipids in cells and tissue became possible, while simultaneous fluorescence imaging enabled cell-type identification in complex tissues. In proof-of-concept experiments, newly synthesized lipids were directly visualized in human-relevant model systems among different cell types, mutation status, differentiation stages, and over time. We identified upregulated lipid metabolism in progranulin-knockdown human induced pluripotent stem cells and in their differentiated microglia cells. Furthermore, we observed that neurons in brain organoids exhibited a significantly lower lipid metabolism compared to astrocytes.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems

Bai, Yeran ; Camargo, Carolina M ; Glasauer, Stella M K ; Gifford, Raymond ; Tian, Xinran ; Longhini, Andrew P ; Kosik, Kenneth S

England: Nature Publishing Group

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