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Functional Repression of Islet-2 by Disruption of Complex with Ldb Impairs Peripheral Axonal Outgrowth in Embryonic Zebrafish

Segawa, Hiroshi ; Miyashita, Toshio ; Hirate, Yoshikazu ; Higashijima, Shin-ichi ; Chino, Naoichi ; Uyemura, Keiichi ; Kikuchi, Yutaka ; Okamoto, Hitoshi

Neuron (Cambridge, Mass.), 2001-05, Vol.30 (2), p.423-436 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Functional Repression of Islet-2 by Disruption of Complex with Ldb Impairs Peripheral Axonal Outgrowth in Embryonic Zebrafish
Autor: Segawa, Hiroshi ; Miyashita, Toshio ; Hirate, Yoshikazu ; Higashijima, Shin-ichi ; Chino, Naoichi ; Uyemura, Keiichi ; Kikuchi, Yutaka ; Okamoto, Hitoshi
Assuntos: Animals ; Axons - physiology ; Binding Sites ; Danio rerio ; Embryo, Nonmammalian - physiology ; Genes, Reporter ; Green Fluorescent Proteins ; Homeodomain Proteins - chemistry ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Islet-2 protein ; LIM protein ; LIM-Homeodomain Proteins ; Luminescent Proteins - analysis ; Luminescent Proteins - genetics ; Mosaicism ; Motor Neurons - physiology ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Neurons, Afferent - physiology ; Oligodeoxyribonucleotides, Antisense - pharmacology ; Protein Biosynthesis ; RNA, Messenger - genetics ; Transcription Factors ; Transcription, Genetic ; Zebrafish - embryology ; Zebrafish - genetics
É parte de: Neuron (Cambridge, Mass.), 2001-05, Vol.30 (2), p.423-436
Notas: ObjectType-Article-2
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Descrição: Islet-2 is a LIM/homeodomain-type transcription factor of the Islet-1 family expressed in embryonic zebrafish. Two Islet-2 molecules bind to the LIM domain binding protein (Ldb) dimers. Overexpression of the LIM domains of Islet-2 or the LIM-interacting domain of Ldb proteins prevented binding of Islet-2 to Ldb proteins in vitro and caused similar in vivo defects in positioning, peripheral axonal outgrowth, and neurotransmitter expression by the Islet-2-positive primary sensory and motor neurons as the defects induced by injection of Islet-2-specific antisense morpholino oligonucleotide. These and other experiments, i.e., mosaic analysis, coexpression of full-length Islet-2, and overexpression of the chimeric LIM domains derived from two different Islet-1 family members, demonstrated that Islet-2 regulates neuronal differentiation by forming a complex with Ldb dimers and possibly with some other Islet-2-specific cofactors.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Functional Repression of Islet-2 by Disruption of Complex with Ldb Impairs Peripheral Axonal Outgrowth in Embryonic Zebrafish

Segawa, Hiroshi ; Miyashita, Toshio ; Hirate, Yoshikazu ; Higashijima, Shin-ichi ; Chino, Naoichi ; Uyemura, Keiichi ; Kikuchi, Yutaka ; Okamoto, Hitoshi

United States: Elsevier Inc

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