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Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium / Lomentospora in Cystic Fibrosis Patients

Martin-Souto, Leire ; Antoran, Aitziber ; Areitio, Maialen ; Aparicio-Fernandez, Leire ; Martín-Gómez, María Teresa ; Fernandez, Roberto ; Astigarraga, Egoitz ; Barreda-Gómez, Gabriel ; Schwarz, Carsten ; Rickerts, Volker ; Hernando, Fernando L ; Rementeria, Aitor ; Buldain, Idoia ; Ramirez-Garcia, Andoni

Journal of fungi (Basel), 2023-01, Vol.9 (2), p.158 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium / Lomentospora in Cystic Fibrosis Patients
Autor: Martin-Souto, Leire ; Antoran, Aitziber ; Areitio, Maialen ; Aparicio-Fernandez, Leire ; Martín-Gómez, María Teresa ; Fernandez, Roberto ; Astigarraga, Egoitz ; Barreda-Gómez, Gabriel ; Schwarz, Carsten ; Rickerts, Volker ; Hernando, Fernando L ; Rementeria, Aitor ; Buldain, Idoia ; Ramirez-Garcia, Andoni
Assuntos: antibody detection ; Antigens ; Chronic infection ; Conidia ; Cystic fibrosis ; Diagnosis ; Hyphae ; immunoassay ; Immunoglobulin G ; Lomentospora ; Multivariate analysis ; Scedosporium ; serological detection ; Software ; Sputum
É parte de: Journal of fungi (Basel), 2023-01, Vol.9 (2), p.158
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The detection of / is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against / in less than 15 min was developed. A crude protein extract from the conidia and hyphae of was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of / in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that / positive sputum, elevated anti- serum IgG and chronic infection were significantly associated with a positive result in DIA, while positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of / in patients with CF.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium / Lomentospora in Cystic Fibrosis Patients

Switzerland: MDPI AG

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