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The formation of persister cells in stationary-phase cultures of Escherichia coli is associated with the aggregation of endogenous proteins

Leszczynska, Daria ; Matuszewska, Ewelina ; Kuczynska-Wisnik, Dorota ; Furmanek-Blaszk, Beata ; Laskowska, Ewa Rohde, Holger

PloS one, 2013-01, Vol.8 (1), p.e54737-e54737 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
The formation of persister cells in stationary-phase cultures of Escherichia coli is associated with the aggregation of endogenous proteins
Autor: Leszczynska, Daria ; Matuszewska, Ewelina ; Kuczynska-Wisnik, Dorota ; Furmanek-Blaszk, Beata ; Laskowska, Ewa
Rohde, Holger
Assuntos: Acetic acid ; Adenosine Triphosphate - metabolism ; Agglomeration ; Aggregates ; Aging ; Analysis ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Apoptosis ; Bacteria ; Bacterial Proteins - metabolism ; Betaine ; Biochemistry ; Biology ; Deoxyribonucleic acid ; DNA ; E coli ; Escherichia coli ; Escherichia coli - cytology ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Gene mutation ; Gene mutations ; Glycerol ; Homeostasis ; Kinases ; Laboratories ; Low concentrations ; Medicine ; Metabolism ; Metabolites ; Mopping ; Mutation ; Oxidation ; Physiology ; Protein biosynthesis ; Protein interaction ; Protein synthesis ; Proteins ; Trehalose
É parte de: PloS one, 2013-01, Vol.8 (1), p.e54737-e54737
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: EL EM DKW. Performed the experiments: DL EM DKW BFB EL. Analyzed the data: DL EM DKW BFB EL. Contributed reagents/materials/analysis tools: DL EM DKW EL. Wrote the paper: EL EM BFB.
Descrição: Persister cells (persisters) are transiently tolerant to antibiotics and usually constitute a small part of bacterial populations. Persisters remain dormant but are able to re-grow after antibiotic treatment. In this study we found that the frequency of persisters correlated to the level of protein aggregates accumulated in E. coli stationary-phase cultures. When 3-(N-morpholino) propanesulfonic acid or an osmolyte (trehalose, betaine, glycerol or glucose) were added to the growth medium at low concentrations, proteins were prevented from aggregation and persister formation was inhibited. On the other hand, acetate or high concentrations of osmolytes enhanced protein aggregation and the generation of persisters. We demonstrated that in the E. coli stationary-phase cultures supplemented with MOPS or a selected osmolyte, the level of protein aggregates and persister frequency were not correlated with such physiological parameters as the extent of protein oxidation, culturability, ATP level or membrane integrity. The results described here may help to understand the mechanisms underlying persister formation.
Editor: United States: Public Library of Science
Idioma: Inglês

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The formation of persister cells in stationary-phase cultures of Escherichia coli is associated with the aggregation of endogenous proteins

Leszczynska, Daria ; Matuszewska, Ewelina ; Kuczynska-Wisnik, Dorota ; Furmanek-Blaszk, Beata ; Laskowska, Ewa Rohde, Holger

United States: Public Library of Science

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