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Expression of ADAMTS4 in Ewing's sarcoma

MINOBE, K ; ONO, R ; HORI, H ; KOMADA, Y ; UCHIDA, A ; HAYASHI, Y ; KITAMURA, T ; NOSAKA, T ; MATSUMINE, A ; SHIBATA-MINOSHIMA, F ; IZAWA, K ; OKI, T ; KITAURA, J ; IINO, T ; TAKITA, J ; IWAMOTO, S

International journal of oncology, 2010-09, Vol.37 (3), p.569-581

Athens: Editorial Academy of the International Journal of Oncology

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Título:
Expression of ADAMTS4 in Ewing's sarcoma
Autor: MINOBE, K ; ONO, R ; HORI, H ; KOMADA, Y ; UCHIDA, A ; HAYASHI, Y ; KITAMURA, T ; NOSAKA, T ; MATSUMINE, A ; SHIBATA-MINOSHIMA, F ; IZAWA, K ; OKI, T ; KITAURA, J ; IINO, T ; TAKITA, J ; IWAMOTO, S
Assuntos: ADAM Proteins - biosynthesis ; ADAM Proteins - genetics ; ADAMTS4 Protein ; Adolescent ; Animals ; Biological and medical sciences ; Cell Line, Tumor ; Diseases of the osteoarticular system ; DNA, Complementary - genetics ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; Medical sciences ; Mice ; NIH 3T3 Cells ; Oncogene Proteins, Fusion - genetics ; Oncogene Proteins, Fusion - metabolism ; Procollagen N-Endopeptidase - biosynthesis ; Procollagen N-Endopeptidase - genetics ; Proto-Oncogene Protein c-fli-1 - genetics ; Proto-Oncogene Protein c-fli-1 - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; RNA-Binding Protein EWS ; Sarcoma, Ewing - enzymology ; Sarcoma, Ewing - genetics ; Sarcoma, Ewing - pathology ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transfection ; Tumors ; Tumors of striated muscle and skeleton ; Up-Regulation
É parte de: International journal of oncology, 2010-09, Vol.37 (3), p.569-581
Descrição: Ewing's sarcoma (EWS) is a malignant bone tumor that frequently occurs in teenagers. Genetic mutations which cause EWS have been investigated, and the most frequent one proved to be a fusion gene between EWS gene of chromosome 22 and the FLI1 gene of chromosome 11. However, a limited numbers of useful biological markers for diagnosis of EWS are available. In this study, we identified ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs) as a possible tumor marker for EWS using the retrovirus-mediated signal sequence trap method. ADAMTS4 is a secreted protein of 837 amino acids with a predicted molecular mass of 98-100 kDa. It is a member of metalloprotease family, is expressed mainly in cartilage and brain, and regulates the degradation of aggrecans. ADAMTS4 has been suggested to be involved in arthritic diseases and gliomas. Herein, we show that ADAMTS4 mRNA was expressed in all primary EWS samples and all EWS-derived cell lines examined, while its expression was detected only in small subpopulations of other solid tumors. Furthermore, ADAMTS4 expression was found to be regulated by EWS-FLI1 fusion gene-dependent manner. We also demonstrated that ADAMTS4 protein was highly expressed in tumor samples of the patients with EWS by using immunohistochemistry. These results suggest that ADAMTS4 is a novel tumor marker for EWS.
Editor: Athens: Editorial Academy of the International Journal of Oncology
Idioma: Inglês

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Expression of ADAMTS4 in Ewing's sarcoma

MINOBE, K ; ONO, R ; HORI, H ; KOMADA, Y ; UCHIDA, A ; HAYASHI, Y ; KITAMURA, T ; NOSAKA, T ; MATSUMINE, A ; SHIBATA-MINOSHIMA, F ; IZAWA, K ; OKI, T ; KITAURA, J ; IINO, T ; TAKITA, J ; IWAMOTO, S

Athens: Editorial Academy of the International Journal of Oncology

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