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6-OHDA activates nuclear factor NF-kB in PC 12 cells

L. B. Lepsch E. M Kawamoto; Carolina Demarchi Munhoz; L. S Lima; C. S Planeta; Cristoforo Scavone; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (20. 2005 Águas de Lindóia, SP)

Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005

Águas de Lindóia, São Paulo Federação de Sociedades de Biologia Experimental 2005

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  • Título:
    6-OHDA activates nuclear factor NF-kB in PC 12 cells
  • Autor: L. B. Lepsch
  • E. M Kawamoto; Carolina Demarchi Munhoz; L. S Lima; C. S Planeta; Cristoforo Scavone; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (20. 2005 Águas de Lindóia, SP)
  • Assuntos: FARMACOLOGIA
  • É parte de: Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Objetivo: 6-hydroxydopamine (6-OHDA) is a neurotoxin that has been extensively used as a model of Parkinson's disease by selective degeneration of nigrostriatal neurons. The 6-OHDA induces apoptosis in the rat phaechromocytoma cell line PC 12. NF-_B is a transcription factor which plays an important role in the control of the apoptotic response. Our aim was to evaluate if 6-OHDA can modulate NF-_B in PC 12 cells. Métodos e Resultados: PC 12 cells which was cultured in dishes were treated with 6-OHDA (100µM) in different times of incubation (1,2, 4,6 and 8 h). Nuclear extracts were isolated and gel mobility shift assay used to measure changes in NF-_B activity. Competition studies with specific and non-specific unlabeled double-strand oligonucleotide and super-shift assays with specific antibodies against NF-_B subunits p50 and p65 were also performed. 6-OHDA induced activation of NF-_B in PC 12 cells after 8 hours of incubation (cont:6051± UA n=3; 6-OHDA:12242±\3= __). This activation starded at 6h and enhanced at 8 h after 6-OHDA incubation. Super-shift assays indicated that the major DNA-protein complex altered by 6-OHDA corresponded to p65/p65 heterodimer. Conclusões: Our results suggest that 6-OHDA induces a time-dependent activation of p65/p65 NF-_B in PC 12 cells
  • Editor: Águas de Lindóia, São Paulo Federação de Sociedades de Biologia Experimental
  • Data de criação/publicação: 2005
  • Formato: res. 13.003.
  • Idioma: Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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