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Formation of Arrayed Droplets by Soft Lithography and Two-Phase Fluid Flow, and Application in Protein Crystallization

Zheng, B. ; Tice, J. D. ; Ismagilov, R. F.

Advanced materials (Weinheim), 2004-08, Vol.16 (15), p.1365-1368 [Periódico revisado por pares]

Weinheim: WILEY-VCH Verlag

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  • Título:
    Formation of Arrayed Droplets by Soft Lithography and Two-Phase Fluid Flow, and Application in Protein Crystallization
  • Autor: Zheng, B. ; Tice, J. D. ; Ismagilov, R. F.
  • Assuntos: Microfluidics ; Protein crystallization ; Soft lithography
  • É parte de: Advanced materials (Weinheim), 2004-08, Vol.16 (15), p.1365-1368
  • Notas: istex:8218CB8482184FCA53E804759B1EA606065C9619
    ark:/67375/WNG-6LRM82DS-K
    This work was supported in part by the NIH (R01 EB001903) and the Beckman Young Investigator Program, and was performed at the MRSEC microfluidic facility funded by the NSF.
    ArticleID:ADMA200400590
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  • Descrição: This paper presents an overview of our recent work on the use of soft lithography and two‐phase fluid flow to form arrays of droplets. The crucial issues in the formation of stable arrays of droplets and alternating droplets of two sets of aqueous solutions include the geometry of the microchannels, the capillary number, and the water fraction of the system. Glass capillaries could be coupled to the PDMS microchannels and droplets could be transferred into glass capillaries for long‐term storage. The arrays of droplets have been applied to screen the conditions for protein crystallization with microbatch and vapor diffusion techniques. Soft lithography and two‐phase fluid flow have been used in order to form arrays of droplets (see Figure). Glass capillaries could be coupled to the poly(dimethylsiloxane) (PDMS) microchannels and droplets could be transferred into glass capillaries for long‐term storage. The arrays of droplets have been applied to screen the conditions for protein crystallization with microbatch and vapor diffusion techniques.
  • Editor: Weinheim: WILEY-VCH Verlag
  • Idioma: Inglês

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