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Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum

Guimarães, Allan Jefferson ; Hamilton, Andrew John ; de M Guedes, Herbert Leonel ; Nosanchuk, Joshua Daniel ; Zancopé-Oliveira, Rosely Maria Nielsen, Kirsten

PloS one, 2008-10, Vol.3 (10), p.e3449-e3449 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum
Autor: Guimarães, Allan Jefferson ; Hamilton, Andrew John ; de M Guedes, Herbert Leonel ; Nosanchuk, Joshua Daniel ; Zancopé-Oliveira, Rosely Maria
Nielsen, Kirsten
Assuntos: Amino Acid Sequence ; Analysis ; Antibodies, Fungal - immunology ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Antigenic determinants ; Antigens ; Antigens, Fungal - chemistry ; Antigens, Fungal - genetics ; Antigens, Fungal - physiology ; Ascomycota ; Aspergillus fumigatus ; Biochemistry ; Biochemistry/Bioinformatics ; Biotechnology/Applied Microbiology ; Catalase ; Catalase - metabolism ; Cell Biology/Cellular Death and Stress Responses ; Cell Biology/Microbial Growth and Development ; Cell surface ; Cell walls ; Computational Biology ; Computational Biology/Evolutionary Modeling ; Enzymes ; Epitopes - immunology ; Fungi ; Glycoproteins - chemistry ; Glycoproteins - genetics ; Glycoproteins - physiology ; Histoplasma - immunology ; Histoplasma - physiology ; Histoplasmosis ; Homology ; Hydrogen peroxide ; Immune system ; Immunodominance ; Immunology ; Immunology/Immune Response ; Immunology/Immunity to Infections ; Infections ; Infectious diseases ; Localization ; Medicine ; Microbiology/Applied Microbiology ; Microbiology/Cellular Microbiology and Pathogenesis ; Microbiology/Medical Microbiology ; Models, Molecular ; Molecular Sequence Data ; Monoclonal antibodies ; Mycoses ; Oxidative Stress ; Pathogenesis ; Phylogeny ; Proteins ; Sequence Alignment ; Studies ; Surface antigens ; Yeast ; Yeasts - immunology
É parte de: PloS one, 2008-10, Vol.3 (10), p.e3449-e3449
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
Conceived and designed the experiments: AJG AH JDN RMZO. Performed the experiments: AJG JDN. Analyzed the data: AJG HLdMG JDN. Contributed reagents/materials/analysis tools: AJG AH JDN RMZO. Wrote the paper: AJG JDN RMZO.
Descrição: Histoplasmosis, due to the intracellular fungus Histoplasma capsulatum, can be diagnosed by demonstrating the presence of antibodies specific to the immunodominant M antigen. However, the role of this protein in the pathogenesis of histoplasmosis has not been elucidated. We sought to structurally and immunologically characterize the protein, determine yeast cell surface expression, and confirm catalase activity. A 3D-rendering of the M antigen by homology modeling revealed that the structures and domains closely resemble characterized fungal catalases. We generated monoclonal antibodies (mAbs) to the protein and determined that the M antigen is present on the yeast cell surface and in cell wall/cell membrane preparations. Similarly, we found that the majority of catalase activity was in extracts containing fungal surface antigens and that the M antigen is not significantly secreted by live yeast cells. The mAbs also identified unique epitopes on the M antigen. The localization of the M antigen to the cell surface of H. capsulatum yeast and the characterization of the protein's major epitopes have important implications since it demonstrates that although the protein may participate in protecting the fungus against oxidative stress it is also accessible to host immune cells and antibody.
Editor: United States: Public Library of Science
Idioma: Inglês

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Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum

Guimarães, Allan Jefferson ; Hamilton, Andrew John ; de M Guedes, Herbert Leonel ; Nosanchuk, Joshua Daniel ; Zancopé-Oliveira, Rosely Maria Nielsen, Kirsten

United States: Public Library of Science

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