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Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori

Sakurai, T ; Nakagawa, T ; Mitsuno, H ; Mori, H ; Endo, Y ; Tanoue, S ; Yasukochi, Y ; Touhara, K ; Nishioka, T

Proceedings of the National Academy of Sciences - PNAS, 2004-11, Vol.101 (47), p.16653-16658 [Periódico revisado por pares]

United States: National Academy of Sciences

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Título:
Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori
Autor: Sakurai, T ; Nakagawa, T ; Mitsuno, H ; Mori, H ; Endo, Y ; Tanoue, S ; Yasukochi, Y ; Touhara, K ; Nishioka, T
Assuntos: Amino Acid Sequence ; Animals ; Antennas ; Baculovirus ; Base Sequence ; Biological Sciences ; BmOR-1 gene ; bombykol ; Bombyx - genetics ; Bombyx - physiology ; Bombyx mori ; chemoreceptors ; Complementary DNA ; DNA, Complementary - genetics ; electroantennography ; electrophysiology ; exons ; Female ; Gene Expression ; genes ; Genes, Insect ; Genomics ; In Situ Hybridization ; Insect antennae ; Insect Proteins - genetics ; Insect Proteins - physiology ; introns ; Male ; Male animals ; messenger RNA ; Molecular Sequence Data ; Moths ; Neurology ; Neurons ; neurophysiology ; Nucleopolyhedrovirus - genetics ; nucleotide sequences ; olfactory receptor 1 ; Oocytes ; Oocytes - metabolism ; Phylogeny ; Receptors ; Receptors, Mating Factor ; Receptors, Odorant - genetics ; Receptors, Odorant - physiology ; Receptors, Peptide - genetics ; Receptors, Peptide - physiology ; Recombination, Genetic ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sex attractants ; sex pheromones ; Transcription Factors - genetics ; Transcription Factors - physiology ; Xenopus laevis
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2004-11, Vol.101 (47), p.16653-16658
Notas: ObjectType-Article-2
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Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AB059431, AB100454, AB105070, and AB101293 for cDNA sequences of BmOR-1, BmOR-2, and BmGαq and genomic DNA sequence of BmOR-1, respectively).
Abbreviations: OR, olfactory receptor; BmOR, Bombyx mori OR; PBP, pheromone-binding protein; DIG, digoxigenin; EAG, electroantennogram; HyNPV, hybrid nuclear polyhedrosis virus.
To whom correspondence should be addressed. E-mail: touhara@k.u-tokyo.ac.jp or nishioka@scl.kyoto-u.ac.jp.
Present address: Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513.
Communicated by Yoshito Kaziro, Kyoto University, Kyoto, Japan, October 13, 2004
Descrição: Sex pheromones released by female moths are detected with high specificity and sensitivity in the olfactory sensilla of antennae of conspecific males. Bombykol in the silkmoth Bombyx mori was the first sex pheromone to be identified. Here we identify a male-specific G protein-coupled olfactory receptor gene, B. mori olfactory receptor 1 (BmOR-1), that appears to encode a bombykol receptor. The BmOR-1 gene is located on the Z sex chromosome, has an eight-exon/seven-intron structure, and exhibits male-specific expression in the pheromone receptor neurons of male moth antenna during late pupal and adult stages. Bombykol stimulation of Xenopus laevis oocytes expressing BmOR-1 and BmGαq elicited robust dose-dependent inward currents on two-electrode voltage clamp recordings, demonstrating that the binding of bombykol to BmOR-1 leads to the activation of a BmGαq-mediated signaling cascade. Antennae of female moths infected with BmOR-1-recombinant baculovirus showed electrophysiological responses to bombykol but not to bombykal. These results provide evidence that BmOR-1 is a G protein-coupled sex pheromone receptor that recognizes bombykol.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori

Sakurai, T ; Nakagawa, T ; Mitsuno, H ; Mori, H ; Endo, Y ; Tanoue, S ; Yasukochi, Y ; Touhara, K ; Nishioka, T

United States: National Academy of Sciences

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