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Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products

Demarque, Daniel P ; Dusi, Renata G ; de Sousa, Francisco D M ; Grossi, Sophia M ; Silvério, Maira R S ; Lopes, Norberto P ; Espindola, Laila S

Scientific reports, 2020-01, Vol.10 (1), p.1051, Article 1051 [Periódico revisado por pares]

England: Nature Publishing Group

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Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products
Autor: Demarque, Daniel P ; Dusi, Renata G ; de Sousa, Francisco D M ; Grossi, Sophia M ; Silvério, Maira R S ; Lopes, Norberto P ; Espindola, Laila S
Assuntos: Aedes - drug effects ; Animals ; Annona - chemistry ; Biological Products - analysis ; Biological Products - isolation & purification ; Biological Products - pharmacology ; Chromatography, Liquid - methods ; Insecticides - analysis ; Insecticides - isolation & purification ; Insecticides - pharmacology ; Larva - drug effects ; Larvicides ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Metabolomics ; Metabolomics - methods ; Natural products ; Plant extracts ; Plant Extracts - pharmacology ; Scientific imaging ; Secondary Metabolism ; Tandem Mass Spectrometry - methods
É parte de: Scientific reports, 2020-01, Vol.10 (1), p.1051, Article 1051
Descrição: Metabolomics is a powerful tool in the analysis and identification of metabolites responsible for biological properties. Regarding natural product chemistry, it constitutes a potential strategy to streamline the classic and laborious process of isolating natural products, which often involves the re-isolation and identification of known compounds. In this contribution, we establish a mass spectrometry-based metabolomics strategy to discover compounds with larvicidal activity against Aedes aegypti. We analyse the Brazilian plant Annona crassiflora using different platforms to annotate the active compounds in different extracts/fractions of various plant parts. The MetaboAnalyst and GNPS platforms, which consider LC-MS and LC-MS/MS data, respectively, were chosen to identify compounds that differentiate active and inactive samples. Bio-guided isolation was subsequently performed to confirm compound activity. Results proved the capacity of metabolomics to predict metabolite differences between active and inactive samples using LC-MS and LC-MS/MS data. Moreover, we discuss the limitations, possibilities, and strategies to have a broad view of vast data.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products

Demarque, Daniel P ; Dusi, Renata G ; de Sousa, Francisco D M ; Grossi, Sophia M ; Silvério, Maira R S ; Lopes, Norberto P ; Espindola, Laila S

England: Nature Publishing Group

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