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Bacterial lipopolysaccharide forms aggregates with apolipoproteins in male and female rat brains after ethanol binges

López-Valencia, L. ; Moya, M. ; Escudero, B. ; García-Bueno, B. ; Orio, L.

Journal of lipid research, 2024-03, Vol.65 (3), p.100509-100509, Article 100509 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Bacterial lipopolysaccharide forms aggregates with apolipoproteins in male and female rat brains after ethanol binges
  • Autor: López-Valencia, L. ; Moya, M. ; Escudero, B. ; García-Bueno, B. ; Orio, L.
  • Assuntos: alcohol ; Animals ; ApoAI ; ApoB ; apolipoprotein ; Apolipoproteins - metabolism ; Apolipoproteins B - metabolism ; Apolipoproteins E - metabolism ; binge drinking ; Brain - metabolism ; Corticosterone - metabolism ; Ethanol ; Female ; Lipid A ; Lipid A - metabolism ; Lipopolysaccharides - metabolism ; Lipopolysaccharides - pharmacology ; LPS ; Male ; neuroinflammation ; Neuroinflammatory Diseases ; Rats ; sex-differences ; TLR4
  • É parte de: Journal of lipid research, 2024-03, Vol.65 (3), p.100509-100509, Article 100509
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: Alcohol binge drinking allows the translocation of bacterial lipopolysaccharide (LPS) from the gut to the blood, which activates the peripheral immune system with consequences in neuroinflammation. A possible access/direct signaling of LPS to/in the brain has not yet been described under alcohol abuse conditions. Apolipoproteins are compounds altered by alcohol with high affinity to LPS which may be involved in its transport to the brain or in its elimination. Here, we explored the expression of small components of LPS, in its free form or bound to apolipoproteins, in the brain of female and male rats exposed to alcohol binges. Animals received ethanol oral gavages (3 g/kg every 8 h) for 4 days. LPS or its components (Lipid A and core), LPS-binding protein, corticosterone, lipoproteins (HDL, LDL), apolipoproteins (ApoAI, ApoB, and ApoE), and their receptors were measured in plasma and/or in nonperfused prefrontal cortex (PFC) and cerebellum. Brain LipidA-apolipoprotein aggregates were determined by Western blotting and confirmed by co-immunoprecipitation. In animals exposed to alcohol binges: 1) plasma LPS-binding protein was elevated in both sexes; 2) females showed elevations in plasma ApoAI and corticosterone levels; 3) Lipid A formed aggregates with ApoAI in the female PFC and with ApoB in males, the latter showing Toll-like receptor 4 upregulation in PFC but not females. These results suggest that small bacterial components are present within the brain, forming aggregates with different apolipoproteins, depending on the sex, after alcohol binge intoxications. Results may have implications for the crosstalk between alcohol, LPS, and neuroinflammation. [Display omitted]
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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