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Human natural killer cells express Na+ channels. A pharmacologic flow cytometric study

Mandler, RN ; Seamer, LC ; Whitlinger, D ; Lennon, M ; Rosenberg, E ; Bankhurst, AD

The Journal of immunology (1950), 1990-03, Vol.144 (6), p.2365-2370 [Periódico revisado por pares]

Bethesda, MD: Am Assoc Immnol

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Título:
Human natural killer cells express Na+ channels. A pharmacologic flow cytometric study
Autor: Mandler, RN ; Seamer, LC ; Whitlinger, D ; Lennon, M ; Rosenberg, E ; Bankhurst, AD
Assuntos: Batrachotoxins - pharmacology ; Biological and medical sciences ; Cell Separation ; Cell Survival ; Cytotoxicity, Immunologic - drug effects ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunity, Cellular - drug effects ; Immunobiology ; In Vitro Techniques ; Killer Cells, Natural - physiology ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; Membrane Potentials - drug effects ; Sodium Channels - drug effects ; Sodium Channels - physiology ; Tetrodotoxin - pharmacology ; Veratridine - pharmacology
É parte de: The Journal of immunology (1950), 1990-03, Vol.144 (6), p.2365-2370
Descrição: Voltage-gated excitability of purified human NK cells was studied by using flow cytometry and the voltage-sensitive dye, oxonol. Highly purified human NK cells (CD16 = 95 +/- 1%) from normal volunteers were prepared by using a negative panning technique. The Na(+)-channel agonists batrachotoxin (BTX) (1 to 4 microM) and veratridine (Ver) (100 to 400 microM) depolarized a population of highly purified human NK cells as determined by flow cytometry. BTX and Ver responses were concentration-, time-, temperature-, and Na(+)-dependent. The Na+ channel antagonist tetrodotoxin (1 microM) blocked BTX and Ver responses. Ver (100 microM) produced significant inhibition of cytotoxicity when purified NK cells were incubated with K562 tumor target cells in a 4-h 51Cr release cytotoxicity assay. The effect was blocked by tetrodotoxin. These results strongly suggest presence of functional Na+ channels in NK cells. Activation of voltage-dependent Na+ channels depolarizes cells and reduces their in vitro cytotoxic function.
Editor: Bethesda, MD: Am Assoc Immnol
Idioma: Inglês

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Human natural killer cells express Na+ channels. A pharmacologic flow cytometric study

Mandler, RN ; Seamer, LC ; Whitlinger, D ; Lennon, M ; Rosenberg, E ; Bankhurst, AD

Bethesda, MD: Am Assoc Immnol

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