Idioma:

Shared and distinct transcriptomic cell types across neocortical areas

Tasic, Bosiljka ; Yao, Zizhen ; Graybuck, Lucas T ; Smith, Kimberly A ; Nguyen, Thuc Nghi ; Bertagnolli, Darren ; Goldy, Jeff ; Garren, Emma ; Economo, Michael N ; Viswanathan, Sarada ; Penn, Osnat ; Bakken, Trygve ; Menon, Vilas ; Miller, Jeremy ; Fong, Olivia ; Hirokawa, Karla E ; Lathia, Kanan ; Rimorin, Christine ; Tieu, Michael ; Larsen, Rachael ; Casper, Tamara ; Barkan, Eliza ; Kroll, Matthew ; Parry, Sheana ; Shapovalova, Nadiya V ; Hirschstein, Daniel ; Pendergraft, Julie ; Sullivan, Heather A ; Kim, Tae Kyung ; Szafer, Aaron ; Dee, Nick ; Groblewski, Peter ; Wickersham, Ian ; Cetin, Ali ; Harris, Julie A ; Levi, Boaz P ; Sunkin, Susan M ; Madisen, Linda ; Daigle, Tanya L ; Looger, Loren ; Bernard, Amy ; Phillips, John ; Lein, Ed ; Hawrylycz, Michael ; Svoboda, Karel ; Jones, Allan R ; Koch, Christof ; Zeng, Hongkui

Nature (London), 2018-11, Vol.563 (7729), p.72-78 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Shared and distinct transcriptomic cell types across neocortical areas
Autor: Tasic, Bosiljka ; Yao, Zizhen ; Graybuck, Lucas T ; Smith, Kimberly A ; Nguyen, Thuc Nghi ; Bertagnolli, Darren ; Goldy, Jeff ; Garren, Emma ; Economo, Michael N ; Viswanathan, Sarada ; Penn, Osnat ; Bakken, Trygve ; Menon, Vilas ; Miller, Jeremy ; Fong, Olivia ; Hirokawa, Karla E ; Lathia, Kanan ; Rimorin, Christine ; Tieu, Michael ; Larsen, Rachael ; Casper, Tamara ; Barkan, Eliza ; Kroll, Matthew ; Parry, Sheana ; Shapovalova, Nadiya V ; Hirschstein, Daniel ; Pendergraft, Julie ; Sullivan, Heather A ; Kim, Tae Kyung ; Szafer, Aaron ; Dee, Nick ; Groblewski, Peter ; Wickersham, Ian ; Cetin, Ali ; Harris, Julie A ; Levi, Boaz P ; Sunkin, Susan M ; Madisen, Linda ; Daigle, Tanya L ; Looger, Loren ; Bernard, Amy ; Phillips, John ; Lein, Ed ; Hawrylycz, Michael ; Svoboda, Karel ; Jones, Allan R ; Koch, Christof ; Zeng, Hongkui
Assuntos: Animals ; Bioinformatics ; Biomarkers - analysis ; Brain ; Brain cells ; Clustering ; Cortex (motor) ; Datasets ; Dissection ; Female ; GABA ; GABAergic Neurons - metabolism ; Gene Expression Profiling ; Gene sequencing ; Glutamatergic transmission ; Glutamic Acid - metabolism ; Labelling ; Male ; Mice ; Motor Cortex - anatomy & histology ; Motor Cortex - cytology ; Motor Cortex - metabolism ; Neocortex ; Neocortex - anatomy & histology ; Neocortex - cytology ; Neocortex - metabolism ; Neurons ; Neurophysiology ; Organ Specificity ; Ribonucleic acid ; RNA ; RNA sequencing ; Sequence Analysis, RNA ; Single-Cell Analysis ; Taxonomy ; Visual cortex ; Visual Cortex - anatomy & histology ; Visual Cortex - cytology ; Visual Cortex - metabolism ; γ-Aminobutyric acid
É parte de: Nature (London), 2018-11, Vol.563 (7729), p.72-78
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Author contributions H.Z. and K.S. conceptualized, and H.Z. and B.T designed and supervised the study. K.S. defined ALM coordinates based on loss-of-function experiments. K.A.S. managed the scRNA-seq pipeline. A.B. and J.Phillips managed pipeline establishment. D.B., J.G., K.L., C.R, M.T and TK.K. performed scRNA-seq. Z.Y, L.TG. and B.T analysed the data with contributions from O.F., O.P., TB., V.M., J.M., A.S. and M.H. I.W., H.A.S. and A.C. provided viral vectors. J.A.H., TN.N., K.E.H. and P.G. conducted viral tracing experiments. P.L., N.D., TC., S.P, E.B., M.K., N.V.S. and D.H. performed single-cell isolation. TN.N. and E.G. performed RNA ISH with RNAscope. L.M. and TL.D. generated transgenic mice. J. Pendergraft provided genotyping. R.L. provided mouse colony management. K.S., M.N.E., S.V. and L.L. provided manually collected cells from ALM. S.M.S. provided program management support. H.Z. and E.L. led the Cell Types Program at the Allen Institute. C.K. and A.R.J. provided funding, institutional support and management. L.TG., Z.Y, TN.N. and B.T prepared the figures. B.T. and H.Z. wrote the manuscript with contributions from C.K., K.S., L.TG., TN.N. and Z.Y., and in consultation with all authors.
Descrição: The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neocortex, here we analysed 23,822 cells from two areas at distant poles of the mouse neocortex: the primary visual cortex and the anterior lateral motor cortex. We define 133 transcriptomic cell types by deep, single-cell RNA sequencing. Nearly all types of GABA (γ-aminobutyric acid)-containing neurons are shared across both areas, whereas most types of glutamatergic neurons were found in one of the two areas. By combining single-cell RNA sequencing and retrograde labelling, we match transcriptomic types of glutamatergic neurons to their long-range projection specificity. Our study establishes a combined transcriptomic and projectional taxonomy of cortical cell types from functionally distinct areas of the adult mouse cortex.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Shared and distinct transcriptomic cell types across neocortical areas

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.