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Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference

Visani, Michela ; de Biase, Dario ; Marucci, Gianluca ; Taccioli, Cristian ; Baruzzi, Agostino ; Pession, Annalisa Monleon, Daniel

PloS one, 2013-01, Vol.8 (1), p.e55314-e55314 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference
Autor: Visani, Michela ; de Biase, Dario ; Marucci, Gianluca ; Taccioli, Cristian ; Baruzzi, Agostino ; Pession, Annalisa
Monleon, Daniel
Assuntos: Adult ; Adults ; Aged ; Analysis ; Antimitotic agents ; Antineoplastic agents ; Attention ; Biology ; Brain ; Brain - metabolism ; Brain cancer ; Brain tumors ; Breast cancer ; Critical point ; Drug resistance ; Epilepsy ; Female ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Neoplastic - genetics ; Gene Expression Regulation, Neoplastic - physiology ; Glioblastoma ; Glioblastoma - metabolism ; Glioblastoma multiforme ; Glioblastomas ; Humans ; Male ; Medicine ; MicroRNA ; MicroRNAs ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; Pathology ; Real-Time Polymerase Chain Reaction ; Reference Values ; Ribonucleic acid ; RNA ; Statistics, Nonparametric ; Studies ; Surgery ; Tumors
É parte de: PloS one, 2013-01, Vol.8 (1), p.e55314-e55314
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Statistical Analysis: CT. Conceived and designed the experiments: DdB MV AP. Performed the experiments: MV DdB. Analyzed the data: DdB MV GM CT AB AP. Contributed reagents/materials/analysis tools: AP AB PERNO Study Group. Wrote the paper: MV DdB AP.
Competing Interests: The authors have declared that no competing interests exist.
Membership of the PERNO Study group is provided in the Acknowledgments.
Descrição: Glioblastoma is the most aggressive brain tumor that may occur in adults. Regardless of the huge improvements in surgery and molecular therapy, the outcome of neoplasia remains poor. MicroRNAs are small molecules involved in several cellular processes, and their expression is altered in the vast majority of tumors. Several studies reported the expression of different miRNAs in glioblastoma, but one of the most critical point in understanding glioblastoma miRNAs profile is the comparison of these studies. In this paper, we focused our attention on the non-neoplastic references used for determining miRNAs expression. The aim of this study was to investigate if using three different non-neoplastic brain references (normal adjacent the tumor, commercial total RNA, and epileptic specimens) could provide discrepant results. The analysis of 19 miRNAs was performed using Real-Time PCR, starting from the set of samples described above and the expression values compared. Moreover, the three different normal RNAs were used to determine the miRNAs profile in 30 glioblastomas. The data showed that different non-neoplastic controls could lead to different results and emphasize the importance of comparing miRNAs profiles obtained using the same experimental condition.
Editor: United States: Public Library of Science
Idioma: Inglês

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Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference

Visani, Michela ; de Biase, Dario ; Marucci, Gianluca ; Taccioli, Cristian ; Baruzzi, Agostino ; Pession, Annalisa Monleon, Daniel

United States: Public Library of Science

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