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Enzyme-linked immunosorbent assay (ELISA) for measles antibody. A comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

DE SOUZA, V. A. U. F ; PANNUTI, C. S ; SUMITA, L. M ; ALBRECHT, P

Revista do Instituto de Medicina Tropical de São Paulo, 1991, Vol.33 (1), p.32-36 [Periódico revisado por pares]

São Paulo: Instituto de Medicina Tropical de São Paulo

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Título:
Enzyme-linked immunosorbent assay (ELISA) for measles antibody. A comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests
Autor: DE SOUZA, V. A. U. F ; PANNUTI, C. S ; SUMITA, L. M ; ALBRECHT, P
Assuntos: Antibodies, Viral - blood ; Biological and medical sciences ; Child ; Complement Hemolytic Activity Assay ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique ; Hemagglutination Inhibition Tests ; Human viral diseases ; Humans ; Infectious diseases ; Measles virus - immunology ; Medical sciences ; Neutralization Tests ; Sensitivity and Specificity ; TROPICAL MEDICINE ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
É parte de: Revista do Instituto de Medicina Tropical de São Paulo, 1991, Vol.33 (1), p.32-36
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copositivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.
Editor: São Paulo: Instituto de Medicina Tropical de São Paulo
Idioma: Inglês;Português

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Enzyme-linked immunosorbent assay (ELISA) for measles antibody. A comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

DE SOUZA, V. A. U. F ; PANNUTI, C. S ; SUMITA, L. M ; ALBRECHT, P

São Paulo: Instituto de Medicina Tropical de São Paulo

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