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Osmostress-induced cell volume loss delays yeast Hog1 signaling by limiting diffusion processes and by Hog1-specific effects

Babazadeh, Roja ; Adiels, Caroline Beck ; Smedh, Maria ; Petelenz-Kurdziel, Elzbieta ; Goksör, Mattias ; Hohmann, Stefan Polymenis, Michael

PloS one, 2013-11, Vol.8 (11), p.e80901-e80901 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Osmostress-induced cell volume loss delays yeast Hog1 signaling by limiting diffusion processes and by Hog1-specific effects
Autor: Babazadeh, Roja ; Adiels, Caroline Beck ; Smedh, Maria ; Petelenz-Kurdziel, Elzbieta ; Goksör, Mattias ; Hohmann, Stefan
Polymenis, Michael
Assuntos: Accumulation ; Active Transport, Cell Nucleus ; Adaptation ; Atom and Molecular Physics and Optics ; Atom- och molekylfysik och optik ; Baking yeast ; Biochemistry and Molecular Biology ; Biofysik ; Biokemi och molekylärbiologi ; Biological activity ; Biophysics ; Cell Biology ; Cell cycle ; Cell Nucleus - metabolism ; Cell size ; Cellbiologi ; Compression ; Cytoplasm ; Cytosol ; Data compression ; Diffusion ; Diffusion processes ; Diffusion rate ; DNA-Binding Proteins - metabolism ; Gene expression ; Glycerin ; Glycerol ; Green Fluorescent Proteins - metabolism ; Hog1 protein ; Kinases ; Localization ; Mitogen-Activated Protein Kinases - metabolism ; Molecular biology ; Nuclear transport ; Osmolarity ; Osmosis ; Osmotic adaptations ; Osmotic Pressure ; Osmotic shock ; Osmotic stress ; Phosphorylation ; Physics ; Protein interaction ; Protein kinase ; Protein kinases ; Protein-protein interactions ; Proteins ; Recombinant Fusion Proteins - metabolism ; Recovery (Medical) ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - cytology ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - metabolism ; Signal processing ; Signal Transduction ; Signal transmission ; Signaling ; Sodium chloride ; Stress ; Stresses ; Transcription Factors - metabolism ; Translocation ; Yeast
É parte de: PloS one, 2013-11, Vol.8 (11), p.e80901-e80901
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Conceived and designed the experiments: RB CBA MS EPK MG SH. Performed the experiments: RB CBA MS EPK. Analyzed the data: RB CBA MS EPK. Wrote the manuscript: RB SH.
Competing Interests: The authors have declared that no competing interests exist.
Current address: Department of Biology, University of Bergen, Bergen, Norway
Descrição: Signal transmission progresses via a series of transient protein-protein interactions and protein movements, which require diffusion within a cell packed with different molecules. Yeast Hog1, the effector protein kinase of the High Osmolarity Glycerol pathway, translocates transiently from the cytosol to the nucleus during adaptation to high external osmolarity. We followed the dynamics of osmostress-induced cell volume loss and Hog1 nuclear accumulation upon exposure of cells to different NaCl concentrations. While Hog1 nuclear accumulation peaked within five minutes following mild osmotic shock it was delayed up to six-fold under severe stress. The timing of Hog1 nuclear accumulation correlated with the degree of cell volume loss and the cells capacity to recover. Also the nuclear translocation of Msn2, the transcription factor of the general stress response pathway, is delayed upon severe osmotic stress suggesting a general phenomenon. We show by direct measurements that the general diffusion rate of Hog1 in the cytoplasm as well as its rate of nuclear transport are dramatically reduced following severe volume reduction. However, neither Hog1 phosphorylation nor Msn2 nuclear translocation were as much delayed as Hog1 nuclear translocation. Our data provide direct evidence that signaling slows down during cell volume compression, probably as a consequence of molecular crowding. Hence one purpose of osmotic adaptation is to restore optimal diffusion rates for biochemical and cell biological processes. In addition, there may be mechanisms slowing down especially Hog1 nuclear translocation under severe stress in order to prioritize Hog1 cytosolic targets.
Editor: United States: Public Library of Science
Idioma: Inglês

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Osmostress-induced cell volume loss delays yeast Hog1 signaling by limiting diffusion processes and by Hog1-specific effects

Babazadeh, Roja ; Adiels, Caroline Beck ; Smedh, Maria ; Petelenz-Kurdziel, Elzbieta ; Goksör, Mattias ; Hohmann, Stefan Polymenis, Michael

United States: Public Library of Science

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