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ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments

Barbosa, Natalia S ; Mendonça, Leila R ; Dias, Marcos V S ; Pontelli, Marjorie C ; da Silva, Elaine Z M ; Criado, Miria F ; da Silva-Januário, Mara E ; Schindler, Michael ; Jamur, Maria C ; Oliver, Constance ; Arruda, Eurico ; daSilva, Luis L P Kuhn, Jens H.

PLoS pathogens, 2018-05, Vol.14 (5), p.e1007047-e1007047 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments
Autor: Barbosa, Natalia S ; Mendonça, Leila R ; Dias, Marcos V S ; Pontelli, Marjorie C ; da Silva, Elaine Z M ; Criado, Miria F ; da Silva-Januário, Mara E ; Schindler, Michael ; Jamur, Maria C ; Oliver, Constance ; Arruda, Eurico ; daSilva, Luis L P
Kuhn, Jens H.
Assuntos: Arbovirus diseases ; Assembly ; Biology and Life Sciences ; Budding ; Bunyaviruses ; Causes of ; Compartments ; Egress ; Endoplasmic reticulum ; Genetic aspects ; Genomes ; Glycoproteins ; Golgi apparatus ; HIV ; Human immunodeficiency virus ; Infections ; Livestock ; Machinery and equipment ; Medical schools ; Microscopy ; Molecular biology ; Morphogenesis ; Particle production ; Proteins ; Replication ; Research and analysis methods ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA viruses ; Supervision ; Virology ; Viruses
É parte de: PLoS pathogens, 2018-05, Vol.14 (5), p.e1007047-e1007047
Notas: new_version
ObjectType-Article-1
SourceType-Scholarly Journals-1
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Current address: Department of Virology and Experimental Therapy LAVITE, Oswaldo Cruz Foundation–FIOCRUZ, Institute Aggeu Magalhães IAM, Recife PE, Brazil
The authors have declared that no competing interests exist.
Descrição: Peribunyaviridae is a large family of RNA viruses with several members that cause mild to severe diseases in humans and livestock. Despite their importance in public heath very little is known about the host cell factors hijacked by these viruses to support assembly and cell egress. Here we show that assembly of Oropouche virus, a member of the genus Orthobunyavirus that causes a frequent arboviral infection in South America countries, involves budding of virus particles toward the lumen of Golgi cisternae. As viral replication progresses, these Golgi subcompartments become enlarged and physically separated from Golgi stacks, forming Oropouche viral factory (Vfs) units. At the ultrastructural level, these virally modified Golgi cisternae acquire an MVB appearance, and while they lack typical early and late endosome markers, they become enriched in endosomal complex required for transport (ESCRT) proteins that are involved in MVB biogenesis. Further microscopy and viral replication analysis showed that functional ESCRT machinery is required for efficient Vf morphogenesis and production of infectious OROV particles. Taken together, our results indicate that OROV attracts ESCRT machinery components to Golgi cisternae to mediate membrane remodeling events required for viral assembly and budding at these compartments. This represents an unprecedented mechanism of how viruses hijack host cell components for coordinated morphogenesis.
Editor: United States: Public Library of Science
Idioma: Inglês

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ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments

Barbosa, Natalia S ; Mendonça, Leila R ; Dias, Marcos V S ; Pontelli, Marjorie C ; da Silva, Elaine Z M ; Criado, Miria F ; da Silva-Januário, Mara E ; Schindler, Michael ; Jamur, Maria C ; Oliver, Constance ; Arruda, Eurico ; daSilva, Luis L P Kuhn, Jens H.

United States: Public Library of Science

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