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P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F

Deak, P. (University of Dundee, UK.) ; Omar, M.M ; Saunders, R.D.C ; Pal, M ; Komonyi, O ; Szidonya, J ; Maroy, P ; Zhang, Y ; Ashburner, M ; Benos, P

Genetics (Austin), 1997-12, Vol.147 (4), p.1697-1722 [Periódico revisado por pares]

United States: Genetics Soc America

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Título:
P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F
Autor: Deak, P. (University of Dundee, UK.) ; Omar, M.M ; Saunders, R.D.C ; Pal, M ; Komonyi, O ; Szidonya, J ; Maroy, P ; Zhang, Y ; Ashburner, M ; Benos, P
Assuntos: Alleles ; Animals ; AUTOSOMES ; Base Sequence ; CARTE GENETIQUE ; CHROMOSOME ; Chromosome Mapping ; CHROMOSOME MAPS ; CHROMOSOMES ; CITOGENETICA ; COMPLEMENTATION ; COMPLEMENTATION GROUPS ; CROMOSOMAS ; CYTOGENETICS ; CYTOGENETIQUE ; CYTOLOGICAL MAPPING ; DNA HYBRIDIZATION ; DNA Transposable Elements ; DNA, Complementary ; DROSOPHILA MELANOGASTER ; Drosophila melanogaster - genetics ; Female ; GENE ; GENE LETAL ; GENES ; GENES LETALES ; Genes, Insect ; Genes, Lethal ; GENETIC MAPPING ; GENETIC MAPS ; GENETIC MARKERS ; GENETICA ; GENETICS ; GENETIQUE ; HIBRIDACION ; HIBRIDACION DE ADN ; HYBRIDATION ; HYBRIDATION D'ADN ; HYBRIDIZATION ; IN SITU HYBRIDIZATION ; INDUCED MUTATION ; Insects ; INSERTION LINES ; INSERTIONAL MUTAGENESIS ; Investigations ; LETHAL GENES ; LINES ; Male ; MAPAS GENETICOS ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; MOLECULAR MAPPING ; MUTACION ; MUTACION INDUCIDA ; Mutagenesis, Insertional ; MUTANT ; MUTANTES ; MUTANTS ; MUTATION ; MUTATION PROVOQUEE ; NUCLEOTIDE SEQUENCE ; OPEN READING FRAMES ; Restriction Mapping ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; SEQUENCE TAGGED SITES ; TRANSPOSABLE ELEMENTS
É parte de: Genetics (Austin), 1997-12, Vol.147 (4), p.1697-1722
Notas: L10
1997082052
ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
Descrição: We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure thought to insert single P elements into vital genes on the third chromosome of Drosophila melanogaster. More than 1200 randomly selected lines were examined by in situ hybridization and 90% found to contain single insertions at sites that mark 89% of all lettered subdivisions of the Bridges' map. A set of chromosomal deficiencies that collectively uncover approximately 25% of the euchromatin of chromosome 3 reveal lethal mutations in 468 lines corresponding to 145 complementation groups. We undertook a detailed analysis of the cytogenetic interval 86E-87F and identified 87 P-element-induced mutations falling into 38 complementation groups, 16 of which correspond to previously known genes. Twenty-one of these 38 complementation groups have at least one allele that has a P-element insertion at a position consistent with the cytogenetics of the locus. We have rescued P elements and flanking chromosomal sequences from the 86E-87F region in 35 lines with either lethal or genetically silent P insertions, and used these as probes to identify cosmids and P1 clones from the Drosophila genome projects. This has tied together the physical and genetic maps and has linked 44 previously identified cosmid contigs into seven "super-contigs" that span the interval. STS data for sequences flanking one side of the P-element insertions in 49 lines has identified insertions in the alphagamma element at 87C, two known transposable elements, and the open reading frames of seven putative single copy genes. These correspond to five known genes in this interval, and two genes identified by the homology of their predicted products to known proteins from other organisms.
Editor: United States: Genetics Soc America
Idioma: Inglês

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P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F

Deak, P. (University of Dundee, UK.) ; Omar, M.M ; Saunders, R.D.C ; Pal, M ; Komonyi, O ; Szidonya, J ; Maroy, P ; Zhang, Y ; Ashburner, M ; Benos, P

United States: Genetics Soc America

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