skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

A semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostaining

Fernezlian, S.M ; Baldavira, C.M ; de Souza, M.L.F ; Farhat, C ; de Vilhena, A.F ; Pereira, J.C.N ; de Campos, J.R.M ; Takagaki, T ; Balancin, M.L ; AbSaber, A.M ; Capelozzi, V.L

Brazilian journal of medical and biological research, 2023-01, Vol.56 (1), p.e12922-e12922 [Periódico revisado por pares]

Associacao Brasileira de Divulgacao Cientifica (ABDC)

Texto completo disponível

Citações Citado por
  • Título:
    A semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostaining
  • Autor: Fernezlian, S.M ; Baldavira, C.M ; de Souza, M.L.F ; Farhat, C ; de Vilhena, A.F ; Pereira, J.C.N ; de Campos, J.R.M ; Takagaki, T ; Balancin, M.L ; AbSaber, A.M ; Capelozzi, V.L
  • Assuntos: BIOLOGY ; Cell cycle ; Chromatin gradient ; Diagnosis ; Diagnostic immunohistochemistry ; Endocrine gland tumors ; Image analysis ; Image processing ; Ki-67 antigen ; MEDICINE, RESEARCH & EXPERIMENTAL ; Methods ; Prognosis ; Pulmonary typical carcinoid tumor ; Stains and staining (Microscopy) ; Thyroid cancer
  • É parte de: Brazilian journal of medical and biological research, 2023-01, Vol.56 (1), p.e12922-e12922
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-3
    content type line 23
    ObjectType-Review-1
  • Descrição: Nuclear proliferation marker MIB-1 (Ki-67) immunohistochemistry (IHC) is used to examine tumor cell proliferation. However, the diagnostic or prognostic value of the Ki-67 nuclear staining intensity and location, defined as nuclear gradient (NG), has not been assessed. This study examined the potential association between Ki-67 NG and cell cycle phases and its effect on the prognosis of pulmonary typical carcinoid (PTC) tumors. We propose a method for classifying the NG of Ki-67 during the cell cycle and compare the results between PTC, pulmonary adenocarcinoma (PAD), and breast ductal carcinoma (BDC). A literature review and objective analysis of IHC-stained paraffin sections were used to determine the Ki-67 labeling index and composed a stratification of the NG into NG1, NG2, and NG3/4 categories. A semi- automated image analysis protocol was established to determine the Ki-67 NG in PTC, PAD, and BDC. High intraobserver consistency and moderate interobserver agreement were achieved in the determination of Ki-67 NG in tumor specimens. NG1 and NG2 were lower in PTC than in PAD and BDC. Cox multivariate analysis of PTC after adjusting for age and number of metastatic lymph nodes showed that Ki- 67 NG1 and NG2 significantly predicted clinical outcomes. The semi-automated method for quantification of Ki-67 nuclear immunostaining proposed in this study could become a valuable diagnostic and prognostic tool in PTC. Key words: Pulmonary typical carcinoid tumor; Chromatin gradient; Cell cycle; Image analysis; Prognosis
  • Editor: Associacao Brasileira de Divulgacao Cientifica (ABDC)
  • Idioma: Inglês;Português
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  2 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.