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[8] End-group analysis using dansyl chloride

Gray, William R.

Methods in Enzymology, 1972, Vol.25, p.121-138 [Periódico revisado por pares]

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Título:
[8] End-group analysis using dansyl chloride
Autor: Gray, William R.
Assuntos: Humans
É parte de: Methods in Enzymology, 1972, Vol.25, p.121-138
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The methods of amino-terminal analysis and sequence determination based on dansyl chloride have application because of the ease with which one can study minute amounts of peptides and proteins. Dansyl chloride is reactive toward a variety of bases. In sequence analysis, the most important of these are the primary and secondary amines present as the terminal amino groups of peptides and proteins. Compared with most other end-group reagents, dansyl chloride is rather susceptible to hydrolysis by water and hydroxyl ions. Many substances greatly quench the fluorescence of dansyl amino acids, including water, acids, and pyridine. Any dansyl chloride that remains is subsequently broken down during the acid hydrolysis; no labeling of newly exposed amino groups occurs under these conditions. The very simple procedure recommended for peptides is usually unsuitable for proteins, mainly because of a need to use much more dansyl chloride, and larger volumes.
Editor: United States: Elsevier Science & Technology
Idioma: Inglês

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