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Small-molecule antioxidant proteome-shields in Deinococcus radiodurans

Daly, Michael J ; Gaidamakova, Elena K ; Matrosova, Vera Y ; Kiang, Juliann G ; Fukumoto, Risaku ; Lee, Duck-Yeon ; Wehr, Nancy B ; Viteri, Gabriela A ; Berlett, Barbara S ; Levine, Rodney L Otto, Michael

PloS one, 2010-09, Vol.5 (9), p.e12570-e12570 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Small-molecule antioxidant proteome-shields in Deinococcus radiodurans
Autor: Daly, Michael J ; Gaidamakova, Elena K ; Matrosova, Vera Y ; Kiang, Juliann G ; Fukumoto, Risaku ; Lee, Duck-Yeon ; Wehr, Nancy B ; Viteri, Gabriela A ; Berlett, Barbara S ; Levine, Rodney L
Otto, Michael
Assuntos: Amino acids ; Antioxidants ; Antioxidants (Nutrients) ; Antioxidants - metabolism ; Armed forces ; Bacteria ; Biochemistry ; Biotechnology/Chemical Biology of the Cell ; Cell Biology/Cellular Death and Stress Responses ; Cell Biology/Chemical Biology of the Cell ; Chemical Biology/Small Molecule Chemistry ; Chromatography ; Cytosol ; Deinococcus - metabolism ; Deinococcus - radiation effects ; Deinococcus radiodurans ; Deoxyribonucleic acid ; Desiccation ; DNA ; DNA Breaks, Double-Stranded - radiation effects ; DNA Repair ; Drying ; E coli ; Enzymes ; Escherichia coli ; Gamma Rays ; Genomes ; Health sciences ; Heart ; Humans ; Ionizing radiation ; Jurkat Cells ; Laboratories ; Lymphocytes ; Lymphocytes T ; Manganese ; Microbiology/Applied Microbiology ; Microbiology/Environmental Microbiology ; Molecular Biology/DNA Repair ; Nucleosides ; Orthophosphate ; Oxidation ; Oxidative stress ; Oxygen ; Pathology ; Peptides ; Phosphates ; Physiology ; Polypeptides ; Proteins ; Proteome - metabolism ; Proteomes ; Radiation protection ; Radiation tolerance ; Radiation-Protective Agents - metabolism ; Reactive oxygen species ; Salmonella ; Salmonella Typhimurium ; Senescence ; T cells ; Ultraviolet radiation
É parte de: PloS one, 2010-09, Vol.5 (9), p.e12570-e12570
Notas: ObjectType-Article-1
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Conceived and designed the experiments: MJD EKG VYM JGK RF DYL NBW GAV BSB RLL. Performed the experiments: MJD EKG VYM JGK RF DYL NBW GAV BSB RLL. Analyzed the data: MJD EKG VYM JGK RF DYL NBW GAV BSB RLL. Wrote the paper: MJD RLL.
Descrição: For Deinococcus radiodurans and other bacteria which are extremely resistant to ionizing radiation, ultraviolet radiation, and desiccation, a mechanistic link exists between resistance, manganese accumulation, and protein protection. We show that ultrafiltered, protein-free preparations of D. radiodurans cell extracts prevent protein oxidation at massive doses of ionizing radiation. In contrast, ultrafiltrates from ionizing radiation-sensitive bacteria were not protective. The D. radiodurans ultrafiltrate was enriched in Mn, phosphate, nucleosides and bases, and peptides. When reconstituted in vitro at concentrations approximating those in the D. radiodurans cytosol, peptides interacted synergistically with Mn(2+) and orthophosphate, and preserved the activity of large, multimeric enzymes exposed to 50,000 Gy, conditions which obliterated DNA. When applied ex vivo, the D. radiodurans ultrafiltrate protected Escherichia coli cells and human Jurkat T cells from extreme cellular insults caused by ionizing radiation. By establishing that Mn(2+)-metabolite complexes of D. radiodurans specifically protect proteins against indirect damage caused by gamma-rays delivered in vast doses, our findings provide the basis for a new approach to radioprotection and insight into how surplus Mn budgets in cells combat reactive oxygen species.
Editor: United States: Public Library of Science
Idioma: Inglês

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Small-molecule antioxidant proteome-shields in Deinococcus radiodurans

Daly, Michael J ; Gaidamakova, Elena K ; Matrosova, Vera Y ; Kiang, Juliann G ; Fukumoto, Risaku ; Lee, Duck-Yeon ; Wehr, Nancy B ; Viteri, Gabriela A ; Berlett, Barbara S ; Levine, Rodney L Otto, Michael

United States: Public Library of Science

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