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Complete Sequencing of the bla NDM-1-Positive IncA/C Plasmid from Escherichia coli ST38 Isolate Suggests a Possible Origin from Plant Pathogens

Sekizuka, Tsuyoshi ; Matsui, Mari ; Yamane, Kunikazu ; Takeuchi, Fumihiko ; Ohnishi, Makoto ; Hishinuma, Akira ; Arakawa, Yoshichika ; Kuroda, Makoto

PloS one, 2011-09, Vol.6 (9) [Periódico revisado por pares]

San Francisco, USA: Public Library of Science

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  • Título:
    Complete Sequencing of the bla NDM-1-Positive IncA/C Plasmid from Escherichia coli ST38 Isolate Suggests a Possible Origin from Plant Pathogens
  • Autor: Sekizuka, Tsuyoshi ; Matsui, Mari ; Yamane, Kunikazu ; Takeuchi, Fumihiko ; Ohnishi, Makoto ; Hishinuma, Akira ; Arakawa, Yoshichika ; Kuroda, Makoto
  • Assuntos: Biology
  • É parte de: PloS one, 2011-09, Vol.6 (9)
  • Notas: Conceived and designed the experiments: MK. Performed the experiments: TS MM KY MK. Analyzed the data: TS FT MK. Contributed reagents/materials/analysis tools: MM KY FT MO AH YA. Wrote the paper: MK.
  • Descrição: The complete sequence of the plasmid pNDM-1_Dok01 carrying New Delhi metallo-β-lactamase (NDM-1) was determined by whole genome shotgun sequencing using Escherichia coli strain NDM-1_Dok01 (multilocus sequence typing type: ST38) and the transconjugant E. coli DH10B. The plasmid is an IncA/C incompatibility type composed of 225 predicted coding sequences in 195.5 kb and partially shares a sequence with bla CMY-2 -positive IncA/C plasmids such as E. coli AR060302 pAR060302 (166.5 kb) and Salmonella enterica serovar Newport pSN254 (176.4 kb). The bla NDM-1 gene in pNDM-1_Dok01 is terminally flanked by two IS 903 elements that are distinct from those of the other characterized NDM-1 plasmids, suggesting that the bla NDM-1 gene has been broadly transposed, together with various mobile elements, as a cassette gene. The chaperonin groES and groEL genes were identified in the bla NDM-1 -related composite transposon, and phylogenetic analysis and guanine-cytosine content (GC) percentage showed similarities to the homologs of plant pathogens such as Pseudoxanthomonas and Xanthomonas spp., implying that plant pathogens are the potential source of the bla NDM-1 gene. The complete sequence of pNDM-1_Dok01 suggests that the bla NDM-1 gene was acquired by a novel composite transposon on an extensively disseminated IncA/C plasmid and transferred to the E. coli ST38 isolate.
  • Editor: San Francisco, USA: Public Library of Science
  • Idioma: Inglês
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