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Circadian integration of glutamatergic signals by little SAAS in novel suprachiasmatic circuits

Atkins, Jr, Norman ; Mitchell, Jennifer W ; Romanova, Elena V ; Morgan, Daniel J ; Cominski, Tara P ; Ecker, Jennifer L ; Pintar, John E ; Sweedler, Jonathan V ; Gillette, Martha U Yamazaki, Shin

PloS one, 2010-09, Vol.5 (9), p.e12612-e12612 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Circadian integration of glutamatergic signals by little SAAS in novel suprachiasmatic circuits
Autor: Atkins, Jr, Norman ; Mitchell, Jennifer W ; Romanova, Elena V ; Morgan, Daniel J ; Cominski, Tara P ; Ecker, Jennifer L ; Pintar, John E ; Sweedler, Jonathan V ; Gillette, Martha U
Yamazaki, Shin
Assuntos: Analysis ; Animals ; Antisera ; Argipressin ; Biological clocks ; Brain ; Brain slice preparation ; c-Fos protein ; Chemical Biology/Protein Chemistry and Proteomics ; Circadian Rhythm ; Circadian rhythms ; Circuits ; Dentistry ; Developmental biology ; Entrainment ; Excitatory Amino Acid Agents - metabolism ; Eye ; Fos protein ; Gastrin ; Gastrin-releasing peptide ; Gastrin-Releasing Peptide - metabolism ; Glutamate ; Glutamatergic transmission ; Glutamic acid receptors (ionotropic) ; Immunohistochemistry ; Information processing ; Information systems ; Intestine ; Laboratory animals ; Light ; Localization ; Mice ; Mice, Inbred C57BL ; Microscopy ; N-Methyl-D-aspartic acid receptors ; Neurons ; Neuropeptides ; Neuropeptides - metabolism ; Neuroscience/Neural Homeostasis ; Neuroscience/Neuronal Signaling Mechanisms ; Neuroscience/Sensory Systems ; Neurosciences ; Nuclei (cytology) ; Peptides ; Physiology ; Physiology/Neural Homeostasis ; Physiology/Neuronal Signaling Mechanisms ; Rats ; Rats, Long-Evans ; Receptors ; Rodents ; Signal Transduction ; Signaling ; Suprachiasmatic nucleus ; Suprachiasmatic Nucleus - physiology ; Synchronism ; Synchronization ; Vasoactive agents ; Vasoactive intestinal peptide ; Vasoactive Intestinal Peptide - metabolism ; Vasopressin
É parte de: PloS one, 2010-09, Vol.5 (9), p.e12612-e12612
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Conceived and designed the experiments: NAJ JWM JVS MUG. Performed the experiments: NAJ EVR JLE. Analyzed the data: NAJ JWM EVR. Contributed reagents/materials/analysis tools: DJM TPC JLE JEP JVS. Wrote the paper: NAJ JWM MUG.
Descrição: Neuropeptides are critical integrative elements within the central circadian clock in the suprachiasmatic nucleus (SCN), where they mediate both cell-to-cell synchronization and phase adjustments that cause light entrainment. Forward peptidomics identified little SAAS, derived from the proSAAS prohormone, among novel SCN peptides, but its role in the SCN is poorly understood. Little SAAS localization and co-expression with established SCN neuropeptides were evaluated by immunohistochemistry using highly specific antisera and stereological analysis. Functional context was assessed relative to c-FOS induction in light-stimulated animals and on neuronal circadian rhythms in glutamate-stimulated brain slices. We found that little SAAS-expressing neurons comprise the third most abundant neuropeptidergic class (16.4%) with unusual functional circuit contexts. Little SAAS is localized within the densely retinorecipient central SCN of both rat and mouse, but not the retinohypothalamic tract (RHT). Some little SAAS colocalizes with vasoactive intestinal polypeptide (VIP) or gastrin-releasing peptide (GRP), known mediators of light signals, but not arginine vasopressin (AVP). Nearly 50% of little SAAS neurons express c-FOS in response to light exposure in early night. Blockade of signals that relay light information, via NMDA receptors or VIP- and GRP-cognate receptors, has no effect on phase delays of circadian rhythms induced by little SAAS. Little SAAS relays signals downstream of light/glutamatergic signaling from eye to SCN, and independent of VIP and GRP action. These findings suggest that little SAAS forms a third SCN neuropeptidergic system, processing light information and activating phase-shifts within novel circuits of the central circadian clock.
Editor: United States: Public Library of Science
Idioma: Inglês

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Circadian integration of glutamatergic signals by little SAAS in novel suprachiasmatic circuits

Atkins, Jr, Norman ; Mitchell, Jennifer W ; Romanova, Elena V ; Morgan, Daniel J ; Cominski, Tara P ; Ecker, Jennifer L ; Pintar, John E ; Sweedler, Jonathan V ; Gillette, Martha U Yamazaki, Shin

United States: Public Library of Science

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