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Distinct functional roles of amphibian (Xenopus laevis) colony‐stimulating factor‐1‐ and interleukin‐34‐derived macrophages

Grayfer, Leon ; Robert, Jacques

Journal of leukocyte biology, 2015-10, Vol.98 (4), p.641-649 [Periódico revisado por pares]

United States: Society for Leukocyte Biology

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  • Título:
    Distinct functional roles of amphibian (Xenopus laevis) colony‐stimulating factor‐1‐ and interleukin‐34‐derived macrophages
  • Autor: Grayfer, Leon ; Robert, Jacques
  • Assuntos: Animals ; antimicrobial response ; Cells, Cultured ; CSF‐1 ; Host Defense & Pathophysiology ; IL‐34 ; Interleukins - immunology ; Macrophage Colony-Stimulating Factor - immunology ; Macrophages - cytology ; Macrophages - immunology ; myelopoiesis ; Ranavirus ; Receptor, Macrophage Colony-Stimulating Factor - immunology ; Xenopus laevis
  • É parte de: Journal of leukocyte biology, 2015-10, Vol.98 (4), p.641-649
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: X. laevis macrophages derived by CSF‐1 and IL‐34 are distinct in their morphology, gene expression, and antimicrobial capacity. Although Mϕ represent the most primordial immune cell subsets, the mechanisms governing their functional heterogeneity remain poorly defined. However, it is well established that the CSF‐1 cytokine contributes to monopoiesis and to this heterogeneity, whereas the unrelated IL‐34 also binds the CSF‐1R toward poorly understood immunologic roles. To delineate the molecular and evolutionary basis behind vertebrate Mϕ functional heterogeneity, we performed comprehensive transcriptional and functional studies of amphibian (Xenopus laevis) BM (in vitro) and PER (in vivo) Mϕ derived by rXlCSF‐1 and rXlIL‐34. Our findings indicate that these amphibian cytokines promote morphologically and functionally distinct Mϕ populations. Mϕ induced by rXlCSF‐1 possess more robust iNOS gene expression, are substantially more phagocytic, display greater NO responses, and exhibit enhanced bactericidal capacities. By contrast, rXlIL‐34‐derived Mϕ express greater levels of Arg‐1 and NADPH oxidase components and possess greater respiratory burst responses. Most notably, whereas CSF‐1 Mϕ are highly susceptible to the emerging FV3 ranavirus, rXlIL‐34 Mϕ exhibit potent antiviral activity against this pathogen, which is dependent on reactive oxygen production. This work marks an advance in our understanding of the possible mechanisms governing vertebrate Mϕ functional heterogeneity.
  • Editor: United States: Society for Leukocyte Biology
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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