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Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens

Rossi, L. (Friedrich Miescher-Institut, Basel, Switzerland.) ; Hohn, B ; Tinlanad, B

Proceedings of the National Academy of Sciences - PNAS, 1996-01, Vol.93 (1), p.126-130 [Periódico revisado por pares]

United States: National Academy of Sciences of the United States of America

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Título:
Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens
Autor: Rossi, L. (Friedrich Miescher-Institut, Basel, Switzerland.) ; Hohn, B ; Tinlanad, B
Assuntos: ADN ; AGROBACTERIUM TUMEFACIENS ; Agrobacterium tumefaciens - genetics ; BACTERIA ; Bacterial Proteins - metabolism ; Base Sequence ; Cell Nucleus - metabolism ; Cellular biology ; DEGRADACION ; DEGRADATION ; Deoxyribonucleases - metabolism ; Deoxyribonucleic acid ; DNA ; DNA Primers - chemistry ; DNA, Bacterial - metabolism ; DNA, Single-Stranded - metabolism ; DNA-Binding Proteins - metabolism ; Flowers & plants ; Genomes ; Integration tables ; Ion Channels ; Molecular Sequence Data ; Molecules ; NICOTIANA TABACUM ; NUCLEASAS ; NUCLEASE ; Plant cells ; Plant DNA ; Plasmids ; PROTEINAS ; PROTEINAS AGLUTINANTES ; PROTEINE ; PROTEINE DE LIAISON ; Proteins ; Recombination, Genetic ; Seedlings ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Virulence
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1996-01, Vol.93 (1), p.126-130
Notas: 9605227
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Descrição: Agrobacterium tumefaciens transfers transferred DNA (T-DNA), a single-stranded segment of its tumor-inducing (Ti) plasmid, to the plant cell nucleus. The Ti-plasmid-encoded virulence E2 (VirE2) protein expressed in the bacterium has single-stranded DNA (ssDNA)-binding properties and has been reported to act in the plant cell. This protein is thought to exert its influence on transfer efficiency by coating and accompanying the single-stranded T-DNA (ss-T-DNA) to the plant cell genome. Here, we analyze different putative roles of the VirE2 protein in the plant cell. In the absence of VirE2 protein, mainly truncated versions of the T-DNA are integrated. We infer that VirE2 protects the ss-T-DNA against nucleolytic attack during the transfer process and that it is interacting with the ss-T-DNA on its way to the plant cell nucleus. Furthermore, the VirE2 protein was found not to be involved in directing the ss-T-DNA to the plant cell nucleus in a manner dependent on a nuclear localization signal, a function which is carried by the NLS of VirD2. In addition, the efficiency of T-DNA integration into the plant genome was found to be VirE2 independent. We conclude that the VirE2 protein of A. tumefaciens is required to preserve the integrity of the T-DNA but does not contribute to the efficiency of the integration step per se.
Editor: United States: National Academy of Sciences of the United States of America
Idioma: Inglês

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Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens

Rossi, L. (Friedrich Miescher-Institut, Basel, Switzerland.) ; Hohn, B ; Tinlanad, B

United States: National Academy of Sciences of the United States of America

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