In vitro and in vivo trypanocidal activity of H2bdtc-loaded solid lipid nanoparticles

• Autor: Carneiro, Zumira A ; Maia, Pedro I da S ; Sesti-Costa, Renata ; Lopes, Carla D ; Pereira, Tatiana A ; Milanezi, Cristiane M ; da Silva, Marcelo A Pereira ; Lopez, Renata F V ; Silva, João S ; Deflon, Victor M
• Pollastri, Michael P.
• Assuntos: Animals ; Chagas Disease ; Disease Models, Animal ; Drug delivery systems ; Female ; Heart - drug effects ; Heart - parasitology ; Lipids ; Lipids - administration & dosage ; Liver - drug effects ; Liver - parasitology ; Medicine and Health Sciences ; Mice ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Parasitemia - drug therapy ; Parasites ; Protozoa ; Thiocarbamates - administration & dosage ; Thiocarbamates - chemistry ; Trypanocidal Agents - administration & dosage ; Trypanocidal Agents - chemistry ; Trypanosoma cruzi ; Trypanosoma cruzi - drug effects
• É parte de: PLoS neglected tropical diseases, 2014-05, Vol.8 (5), p.e2847
• Notas: ObjectType-Article-1
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  Conceived and designed the experiments: ZAC PIdSM RSC CDL TAP CMM MAPdS RFVL JSS VMD. Performed the experiments: ZAC PIdSM RSC CDL TAP CMM MAPdS. Analyzed the data: ZAC PIdSM RSC CDL TAP CMM MAPdS RFVL JSS VMD. Contributed reagents/materials/analysis tools: ZAC PIdSM TAP MAPdS RFVL VMD JSS. Wrote the paper: ZAC PIdSM TAP.
  The authors have declared that no competing interests exist.
  
• Descrição: The parasite Trypanosoma cruzi causes Chagas disease, which remains a serious public health concern and continues to victimize thousands of people, primarily in the poorest regions of Latin America. In the search for new therapeutic drugs against T. cruzi, here we have evaluated both the in vitro and the in vivo activity of 5-hydroxy-3-methyl-5-phenyl-pyrazoline-1-(S-benzyl dithiocarbazate) (H2bdtc) as a free compound or encapsulated into solid lipid nanoparticles (SLN); we compared the results with those achieved by using the currently employed drug, benznidazole. H2bdtc encapsulated into solid lipid nanoparticles (a) effectively reduced parasitemia in mice at concentrations 100 times lower than that normally employed for benznidazole (clinically applied at a concentration of 400 µmol kg(-1) day(-1)); (b) diminished inflammation and lesions of the liver and heart; and (c) resulted in 100% survival of mice infected with T. cruzi. Therefore, H2bdtc is a potent trypanocidal agent.
  
• Editor: United States: Public Library of Science
  
• Idioma: Inglês