Idioma:

Purification of African trypanosomes can cause biochemical changes in the parasites

Lonsdale-Eccles, J.D ; Grab, D.J

The Journal of Protozoology, 1987-11, Vol.34 (4), p.405-408 [Revista revisada por pares]

Oxford, UK: Blackwell Publishing Ltd

Texto completo disponible

Citas Citado por

Acciones

Título:
Purification of African trypanosomes can cause biochemical changes in the parasites
Autor: Lonsdale-Eccles, J.D ; Grab, D.J
Materias: ADENOSINE TRIPHOSPHATE ; Adenosine Triphosphate - metabolism ; ADENOSINTRIFOSFATO ; Animals ; BIOCHEMISTRY ; Biochemistry. Physiology. Immunology. Molecular biology ; BIOCHIMIE ; Biological and medical sciences ; BIOQUIMICA ; BLOOD ; Centrifugation ; Centrifugation, Isopycnic ; Chromatography, DEAE-Cellulose ; Endopeptidases - metabolism ; EPURATION ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Phospholipases - metabolism ; Protozoa ; PURIFICACION ; PURIFICATION ; SANG ; SANGRE ; TRYPANOSOMA ; TRYPANOSOMA BRUCEI ; Trypanosoma brucei brucei - enzymology ; Trypanosoma brucei brucei - isolation & purification ; Trypanosoma brucei brucei - metabolism ; TRYPANOSOMA CONGOLENSE ; Trypanosoma congolense - enzymology ; Trypanosoma congolense - isolation & purification ; Trypanosoma congolense - metabolism ; Trypanosoma vivax ; Variant Surface Glycoproteins, Trypanosoma - metabolism
Es parte de: The Journal of Protozoology, 1987-11, Vol.34 (4), p.405-408
Notas: 881066988
L72
We gratefully acknowledge George W. N. Mpimbaza and Yasmin Verjee for their excellent technical help during this study and Lucy Thairo for typing this manuscript. This is ILRAD publication number 494.
ArticleID:JEU405
istex:5783541C642B36E1007C9AD3D35DF3A5CD04EC5B
ark:/67375/WNG-9N65TB7C-Z
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descripción: Bloodstream forms of African trypanosomes are routinely purified from blood components by a combination of centrifugation and chromatography on DEAE cellulose at pH 8.0. Here we report that the nonphysiological conditions used for DEAE chromatography of the parasites result in changes in the ATP levels of the trypanosomes and an enhanced release from the parasites of proteins such as variable surface glycoprotein, peptidase, and phospholipase. Some of these changes can be reduced by the addition of nucleosides to the elution buffer and, after the elution of the parasites, by immediate readjustment of the external pH to the normal physiological level of blood (pH 7.4).
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglés

Enlaces

Volver a la lista de resultados

Anterior Resultado 5 Siguiente Ir a la siguiente página

Realización: Logos de Redes Sociales:

Purification of African trypanosomes can cause biochemical changes in the parasites

Lonsdale-Eccles, J.D ; Grab, D.J

Oxford, UK: Blackwell Publishing Ltd

Buscando en bases de datos remotas, por favor espere