skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Identification of the Xenopus DNA2 protein as a major nuclease for the 5' arrow right 3' strand-specific processing of DNA ends

Liao, Shuren ; Toczylowski, Thomas ; Yan, Hong

Nucleic acids research, 2008-11, Vol.36 (19), p.6091-6100 [Periódico revisado por pares]

Texto completo disponível

Citações Citado por
  • Título:
    Identification of the Xenopus DNA2 protein as a major nuclease for the 5' arrow right 3' strand-specific processing of DNA ends
  • Autor: Liao, Shuren ; Toczylowski, Thomas ; Yan, Hong
  • Assuntos: Xenopus laevis
  • É parte de: Nucleic acids research, 2008-11, Vol.36 (19), p.6091-6100
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    content type line 23
    ObjectType-Feature-2
  • Descrição: The first step of homology-dependent DNA double-strand break (DSB) repair is the 5' strand-specific processing of DNA ends to generate 3' single-strand tails. Despite extensive effort, the nuclease(s) that is directly responsible for the resection of 5' strands in eukaryotic cells remains elusive. Using nucleoplasmic extracts (NPE) derived from the eggs of Xenopus laevis as the model system, we have found that DNA processing consists of at least two steps: an ATP-dependent unwinding of ends and an ATP-independent 5' arrow right 3' degradation of single-strand tails. The unwinding step is catalyzed by DNA helicases, the major one of which is the Xenopus Werner syndrome protein (xWRN), a member of the RecQ helicase family. In this study, we report the purification and identification of the Xenopus DNA2 (xDNA2) as one of the nucleases responsible for the 5' arrow right 3' degradation of single-strand tails. Immunodepletion of xDNA2 resulted in a significant reduction in end processing and homology-dependent DSB repair. These results provide strong evidence that xDNA2 is a major nuclease for the resection of DNA ends for homology-dependent DSB repair in eukaryotes.
  • Idioma: Inglês
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.